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响应表面优化用于从Gossypium植物园和Solanum melongena土壤中分离的Enterococcus faecium和Stutzerimonas stutzeri的细胞酶生产

Mohammedfaizan Shaikh¹, Naureenbanu Makarani¹, Krishan Kumar²

¹Department of Life Science, Parul Institute of Applied Sciences, Parul University, Waghodia, Vadodara, 391760, Gujarat, India.

Scientific reports

|July 5, 2025

概括

研究人员发现了两个产生细胞酶的细菌,即Enterococcus faecium和Stutzerimonas stutzeri. 这些酶有效地将生物质转化为生物乙醇的糖,可以用于织和造纸行业.

科学领域:

生物技术是生物技术.
微生物学微生物学
生物化学生物化学

背景情况:

红纤维素生物质是化石燃料的可持续替代品.
细胞酶对于将纤维素转化为生物乙醇生产的可发酵糖至关重要.
高效的纤维素生产是各种工业应用的关键.

研究的目的:

选和识别能够产生细胞酶酶的中性菌细菌.
使用统计方法优化细胞酶 (CMCase) 生产.
评估这些酶在工业应用中的潜力,如生物乙醇生产和织品生物抛光.

主要方法:

从CMC亚格板上的土壤样本中选细菌分离物.
使用标准微生物学技术识别有前途的分离物.
使用一次一个因素 (OFAT) 和响应表面方法 (RSM) 与中央复合设计 (CCD) 的CMCase生产的优化.
对棉织物的酶活性测定和应用测试.

主要成果:

鉴定出了两种中性菌的细菌分离物,分别是Enterococcus faecium (BS5) 和Stutzerimonas stutzeri (CS7).这些细菌分离物中,有两种中性菌分离物,分别是Enterococcus faecium (BS5) 和Stutzerimonas stutzeri (CS7).
从Stutzerimonas stutzeri. 报告了一种新型的中性细胞酶.
优化的CMCase生产给BS5带来了20.4U/mL和CS7带来了24.08U/mL的产量,这代表了显著的增长.
原始酶有效地生物抛光棉织物,减少表面纤维.

结论:

菌 (Enterococcus faecium) 和菌 (Stutzerimonas stutzeri) 是中性细胞酶的强有力的来源.
优化生产显著提高了酶产量.
这些酶显示出对工业应用的希望,包括生物乙醇生产,织品生物抛光和纸张脱墨等.

关键词:

碳甲基纤维素 (CMC) 是一种碳甲基纤维素.细胞质细胞质细胞质.红纤维素生物质的生物质.介面性细菌是一种介面性细菌,介面性细菌是介面性细菌.响应表面的方法响应表面方法这就是β-glucosidase.

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