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在胚胎发育期间,ICR小鼠的胎盘结构和基因表达的性别差异在胚胎发育期间
Xinyi Man1, Noriyoshi Usui2,3,4,5,6, Miyuki Doi1,7
1Department of Neuroscience and Cell Biology, Graduate School of Medicine, The University of Osaka, 2-2 Yamadaoka, Suita, 565-0871, Osaka, Japan.
Scientific reports
|July 5, 2025
概括
雌性ICR小鼠在妊娠中期显示出更大的胎盘结区 (JZ) 和更高的JZ基因表达. 胎盘发育中的这些性别差异是特定于ICR小鼠菌株的.
科学领域:
- 生殖生物学 生殖生物学
- 发育生物学是发展生物学.
- 遗传学 遗传学 是一个
背景情况:
- 胎盘对于胎儿发育,营养交换和免疫相互作用至关重要.
- 鼠胎的胎盘发育是广泛使用的模型,但对菌株特定的性别差异尚未完全理解.
研究的目的:
- 为了研究ICR小鼠胎盘发育的性别差异.
- 分析从胚胎日 (E) 8.5到E18.5.5的结区 (JZ) 和迷宫 (LAB) 形态和基因表达.
主要方法:
- 在雄性和雌性ICR小鼠中对胎盘形态 (JZ和LAB区域) 的比较分析.
- 在妊娠中期对JZ相关基因的基因表达分析 (E14.5).
- 与C57BL/6J小鼠进行比较,以确定菌株特定的模式.
主要成果:
- 在E14.5时,雌性ICR小鼠表现出明显更大的JZ面积和相对于胎盘总面积 (JZ + LAB) 的JZ比例.
- 在女性ICR胎盘中观察到与JZ相关的基因表达水平更高,位于E14.5.5.
- 在C57BL/6J小鼠中,没有发现胎盘形态或基因表达的显著性别差异.
结论:
- 在ICR小鼠中,胎盘JZ的雌性偏差扩张发生在妊娠中期.
- 取决于性别的胎盘发育模式是菌株特定的,这突显了研究中使用的小鼠菌株的重要性.
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