开发了Tinospora cordifolia的配方,确定其活性成分,并评估其对乳腺癌细胞系的抗癌作用
Muhasina K M1,2, Md Ekhtiar Uddin3, Ravi Naik Mundavath4
1Department of Pharmacognosy, JSS College of Pharmacy, Ooty, Tamilnadu, 643001, India. km.muhasina@gmail.com.
Medical oncology (Northwood, London, England)
|July 5, 2025
概括
来自Tinospora cordifolia的一种新型纳米脂质体配方显示了对乳腺癌的强有力的抗癌作用. 这种绿色纳米技术方法有效地抑制了癌细胞的生长和迁移,提供了一个有前途的治疗候选者.
科学领域:
- 植物化学和纳米技术
- 药理学和癌症生物学 癌症生物学
背景情况:
- Tinospora cordifolia是一种药用植物,具有公认的药理性质.
- 乳腺癌仍然是一个重大的全球健康挑战,需要新的治疗策略.
研究的目的:
- 开发和评估Tinospora cordifolia的纳米脂质体配方,以对抗乳腺癌细胞系的抗癌疗效.
- 调查作用的潜在机制,包括细胞毒性,迁移抑制,亡诱导和活性氧物种 (ROS) 生成.
主要方法:
- 从T. cordifolia中提取生物活性化合物的绿色提取.
- 开发和描述纳米-脂质体配方 (颗粒大小,通过FESEM的形态学).
- 植物化学分析 (GC-MS),在分子对接 (Topoisomerase IIα) 和体外测试 (MTT,划痕,亡,免疫细胞化学,ROS量化).
主要成果:
- 纳米脂质体配方的颗粒大小为153nm.
- GC-MS确定了35种植物成分; 葡萄糖素通过分子对接显示出强烈的Topoisomerase IIα抑制.
- 该配方表现出剂量依赖的细胞毒性,抑制细胞迁移,诱导亡,并在MCF-7和MDA MB 231细胞中增加ROS生成.
- 亡与改变的Bcl-2,cytochrome-C和caspase-3表达有关.
结论:
- 基于T. cordifolia的纳米脂质体配方显示出针对乳腺癌的显著抗癌潜力.
- 绿色纳米技术,计算方法和基于细胞的测试的整合对于识别生物活性化合物是有效的.
- 该配方需要进一步进行临床前研究,作为潜在的乳腺癌治疗剂.
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