相关实验视频
Updated: Sep 16, 2025

08:26
Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
Published on: February 14, 2020
7.1K
与料摄入和温度调节相关的基因和蛋白质表达的变化在被不同剂量混合Eimeria sppp挑战的肉中
Janghan Choi1, Jihwan Lee2, Woo Kyun Kim2
1Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA.
Poultry science
|July 6, 2025
概括
brojlers 中Eimeria感染显著降低了料摄入量和核心体温. 这种寄生虫感染改变了与食欲调节和温度调节相关的基因和蛋白质表达.
科学领域:
- 鸟类健康 鸟类健康
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
背景情况:
- 埃梅里亚 spp. 的种类. 是重要的原生虫寄生虫,在家禽中引起菌病.
- 菌病会影响肉的表现,包括料摄入量和体温.
- 了解Eimeria感染的分子机制对于有效的疾病管理至关重要.
研究的目的:
- 调查Eimeria感染对基因和蛋白质表达的影响,与肉的料摄入量和核心体温有关.
- 为了确定肉对Eimeria的剂量依赖反应,关于分子标记物的挑战.
主要方法:
- 这是一项完全随机的设计研究,涉及750只15天大的公.
- 建立了五个实验组,包括一个不受挑战的对照组和四个增加剂量的Eimeria spp. (E. acervulina,E. maxima,E. tenella) 这三种类型的植物.
- 评估了大脑,十二指肠和阴中的料摄入量,核心体温以及特定基因 (AGRP,IL18,MR,POMC,CCK,motilin,NPY,NPYY1,CIRBP,HIF1A,HSP70) 的相对mRNA/蛋白质表达.
主要成果:
- 随着Eimeria spp.的剂量增加,料摄入量和核心体温线性下降.
- 调节食欲的基因 (AGRP,POMC,MR,CCK,motilin,NPY) 的表达在大脑和消化道中普遍下调.
- 与温度调节相关的基因 (CIRBP,HIF1A,HSP70) 的表达在十二指肠上升调节.
- 血清中CCK蛋白度在Eimeria剂量较高时线性增加.
结论:
- 艾梅里亚感染通过减少料摄入量和核心体温,显著影响肉生理学.
- 这种寄生虫调节了参与食欲调节和温度调节的关键基因和蛋白质的表达.
- 这些分子变化突出了在肉中感染Eimeria期间复杂的宿主-寄生虫相互作用.
更多相关视频
06:57Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
27.1K
06:38In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth
Published on: September 20, 2021
2.5K
相关概念视频
Stringent Response in E. coli
60
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
60
Background and Environment Affect Phenotype
6.7K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.7K
Cell Specific Gene Expression
14.0K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.0K