电子烟使用者的唾液中烯基醇的定量分析,采用气相色谱-质谱法
Mei-Kuen Tang1, Steven G Carmella2, Yingchun Zhao2
1Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA; Graduate Program in Molecular Pharmacology and Therapeutics, University of Minnesota, Minneapolis, MN 55455, USA.
概括
一种新方法量化了唾液中的烯基醇,这是电子烟使用的潜在生物标志物. 这种验证的测试可以将电子烟使用者与非使用者区分开来,有助于未来对vaping的临床研究.
科学领域:
- 生物标志物和分析化学
- 公共卫生和毒理学
背景情况:
- 电子烟的使用普遍,需要可靠的方法来评估暴露.
- 唾液生物标志物提供了一种非侵入性的方法来监测尼古丁和相关化合物暴露.
研究的目的:
- 开发和验证一种简单的测定方法,用于量化人类唾液中的乙醇 (PG).
- 评估唾液PG水平作为电子烟消费的潜在生物标志物.
主要方法:
- 根据编程和非编程的协议,从电子烟使用者,香烟吸烟者和非使用者收集唾液样本.
- 在衍生后,用气色谱-质谱法 (GC-MS) 量化烯基醇.
- 二醇-d8作为准确量化的内部标准.
主要成果:
- 在电子烟使用者和非使用者之间观察到明显升高的唾液PG水平.在两种协议中,与非使用者相比,电子烟使用者观察到明显升高的唾液PG水平.
- 在电子烟使用者中,编程使用导致更高的PG水平 (269 ± 319 μg/mL) 比非编程使用 (4.94 ± 8.68 μg/mL).
- 吸烟者也比非吸烟者显示出更高的PG水平,但通常低于电子烟使用者.
结论:
- 经过验证的GC-MS试验有效量化了唾液中的烯基醇.
- 唾液PG是一种有前途的生物标志物,用于识别电子烟的使用.
- 这种测试为未来的临床研究提供了有价值的工具,研究电子烟的暴露和影响.
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