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微生物在现场合成尺寸受控的梅利-多酸纳米球,用于输送等离子体

Xueyu Fan¹, Shuo Wang², Huifu Chi²

¹State Key Laboratory of New Pharmaceutical Preparations and Excipients, Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, People's Republic of China; Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

International journal of biological macromolecules

|July 6, 2025

概括

研究人员在E. coli中设计了装载有梅利丁 (Mel) 的聚氨基酸 (PHA) 纳米球. 这些新型纳米载体有效地将CRISPR等离子体输送到细胞中,增强基因编辑传递.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
分子生物学分子生物学

背景情况:

梅利丁 (Mel) 是一种具有药物输送潜力的两性阴性.
梅尔的稳定性和交付效率是有限的.
聚胺酸 (PHA) 纳米球为药物输送提供了一个有前途的平台.

研究的目的:

开发一种稳定高效的纳米载体,用于梅利丁 (Mel) 和CRISPR等离子体.
为了设计大肠杆菌以生产大小受控的Mel载荷PHA纳米球.
为了评估Mel-PHA纳米载体用于CRISPR等离子体输入细胞.

主要方法:

在大肠杆菌中,梅利丁 (Mel) 与PHA颗粒相关蛋白 (PhaP) 的遗传融合.
控制尺寸的Mel载荷PHA纳米球的生产和净化 (约. 在100 nm) 的距离.
在优化的条件下 (37°C,pH 5.35,1小时,质量比为2:1) 将CRISPR/Cas12a等离子体吸附到Mel-PHA纳米球上.

主要成果:

工程化大肠杆菌产生了具有可控尺寸和高Mel负载 (9.9μmol/L) 的Mel-PHA纳米球.
梅尔-PHA纳米球有效地吸附了CRISPR/Cas12a等离子体.
梅尔-PHA@CRISPR纳米载体在体外 (24小时在FBS) 显示稳定性,并通过HEK 293 A细胞有效内化.

结论:

一个自组装,大小控制的纳米载体系统,用于CRISPR等离子体的输送,在大肠杆菌中成功开发出来.
梅尔-PHA纳米载体增强了梅尔和CRISPR等离子体的稳定性和传递效率.
这个平台有潜力推进基因编辑技术.

关键词:

在现场合成.一个小船在里面.在PHA纳米球上.

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