相关实验视频

Updated: Sep 16, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

转录组分析揭示了中潜在的新型circRNA,的进展

Guanying Nie¹, Zixuan Ru², Wei Zhang¹

¹Key Lab of Etiology and Epidemiology, National Health Commission & Education Bureau of Heilongjiang Province (23618504), Key Laboratory of Trace Elements and Human Health, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, China.

|July 6, 2025

概括

这项研究揭示了一种新的circRNA-miR-IGF1轴,该轴因缺而参与的发展. 这一途径,包括mmu-circ-0000554和胰岛素类生长因子1 (IGF1),可能为提供新的诊断和治疗标.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

,通常是由缺乏引起的,提出了当前治疗无法恢复甲状腺功能的挑战.
圆形RNA (circRNA) 分子在病原发生中的作用在很大程度上仍未被探索.

研究的目的:

为了研究circRNA分子在的发展中的参与.
为了确定与缺乏引起的关联的潜在分子机制和生物标志物.

主要方法:

建立一种小鼠模型,用于缺乏引起的.
转录基因测序用于分析基因表达特征.
使用实时定量PCR,西部斑块和免疫组织化学验证.

主要成果:

转录基因分析发现囊中上调调的胰岛素样生长因子1 (IGF1),富含Ras,MAPK和PI3K-Akt通路.
一个涉及mmu-circ-0000554/hsa-circ-0030239-miR-363-5p-IGF1的新轴被确定.
在组织中,mmu-circ-0000554和IGF1的表达增加,并随着补充而逆转;miR-363-5p显示逆调节.

结论:

一个新的circRNA-miR-IGF1轴 (mmu-circ-0000554/hsa-circ-0030239-miR-363-5p-IGF1) 可能在的进展中发挥重要作用.
这些已识别的分子,包括hsa-circ-0020309,IGF1和miR-363-5p,显示出对患者的诊断生物标志物的潜力.
这个轴代表了治疗的潜在治疗目标.

关键词:

痛风 (Goiter) 是一种疾病.Hsa-circ-0030239 在线观看在线观看胰岛素类生长因子 1 1 缺乏症是因为缺少.在米R-363-5p.在Muu-circ-0000554中使用.

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

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