生物电化学降解pyruvate到d-乳酸盐通过工程化Shewanella oneidensis MR-1的工程化
Soshi Taguchi1, Atsumi Hirose1, Atsushi Kouzuma1
1School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Journal of bioscience and bioengineering
|July 6, 2025
概括
谢瓦内拉oneidensisMR-1利用电极电子进行酸盐的降解为d-乳酸盐. 这一过程涉及内膜和NADH依赖的通路,扩大电发酵应用.
科学领域:
- 微生物的电化学
- 代谢工程是代谢工程.
- 细胞外电子转移 (EET) 是一种
背景情况:
- 谢瓦内拉 (Shewanella oneidensis) MR-1是电发酵 (EF) 的模型生物,因为它具有细胞外电子转移 (EET) 能力.
- 在从电极吸收电子时MR-1所使用的细胞内代谢途径尚未完全理解.
研究的目的:
- 为了研究在Shewanella oneidensis MR-1中从阴极吸收电子时的pyruvate的代谢命运.
- 为了阐明参与阴极驱动的酸盐减少的特定酶和途径.
主要方法:
- 使用了一种Shewanella oneidensisMR-1衍生物,缺乏甲酸脱酶 (ΔFDH).
- 在开放电路 (OC) 和封闭电路 (CC) 条件下与静止电极 (-0.36V与SHE) 进行化.
- 进行了比较代谢分析和基因淘汰实验.
主要成果:
- 与OC条件相比,在CC条件下,ΔFDH产生的d-乳酸盐量明显高.
- 从阴极衍生的电子被用来使pyruvate减少为d-乳酸盐.
- Dld (依赖子) 和LdhA (依赖NADH) 几乎均为依赖阴极的d-乳酸盐生产贡献.
结论:
- Shewanella oneidensis MR-1采用内膜子介导和NADH介导的氧化还原反应来从电极转移电子到pyruvate.
- 这些发现凸显了MR-1在各种电发酵过程中的多功能性.
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