Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Histone Modification02:32

Histone Modification

3.6K
3.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.5K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Uniform zinc oxide nanowire arrays grown on nonepitaxial surface with general orientation control.

Nano letters·2013
Same author

[American head and neck surgery progress of in 2012].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2013
Same author

A compact thermo-optical multimode-interference silicon-based 1 × 4 nano-photonic switch.

Optics express·2013
Same author

Experimental demonstration of 110-Gb/s unsynchronized band-multiplexed superchannel coherent optical OFDM/OQAM system.

Optics express·2013
Same author

Potentially functional variants of p14ARF are associated with HPV-positive oropharyngeal cancer patients and survival after definitive chemoradiotherapy.

Carcinogenesis·2013
Same author

Enhanced molecular transport in hierarchical silicalite-1.

Langmuir : the ACS journal of surfaces and colloids·2013

相关实验视频

Updated: Sep 16, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K

无损变异质子修饰分析系统 (LAHMAS)

Zachary J Kauffman1, Kevin Koesser2, Kyle T Helzer3

  • 1Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA. jsperger@medicine.wisc.edu.

Lab on a chip
|July 7, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种用于小型化生物测试的新型微流体系统,提高了灵敏度并减少了样本损失. 无损变异的质子修饰分析系统 (LAHMAS) 能够从罕见细胞中准确地定制染色质概况.

更多相关视频

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

29.5K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K

相关实验视频

Last Updated: Sep 16, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

29.5K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.3K

科学领域:

  • 生物技术是生物技术.
  • 微流体学 微流体学
  • 分子生物学分子生物学

背景情况:

  • 使用微流体微小化生物试验可提高灵敏度和吞吐量,但面临诸如蒸发和分析物损失等挑战.
  • 独家液体排斥 (ELR) 通过使用油相来保护水体量并防止表面结合来解决这些问题.
  • CUT&Tag是一种复杂的多步测试,可以从小型化中获益,以提高效率和减少样本要求.

研究的目的:

  • 开发一种新的微流体平台,用于缩小CUT&Tag试验.
  • 为了在微流体系统中实现无损液体处理,利用排他性液体排斥 (ELR) 和基于排斥的样品制备 (ESP).
  • 创建一个能够从低输入和罕见细胞样本准确绘制染色质概况的系统.

主要方法:

  • 开发了一个微流体平台,使用PDMS-silane处理的玻璃表面浸泡在油中.
  • 综合排斥液体 (ELR) 和基于排斥的样品制备 (ESP) 原则.
  • 利用该平台进行小型化的CUT&Tag测试,命名为无损变异质子修饰分析系统 (LAHMAS).

主要成果:

  • 拉哈马斯平台证明了无损液体处理,并防止了样品的蒸发.
  • 实现了低至100个细胞的细胞输入的有效处理.
  • 与宏观规模的CUT&Tag测定相比,观察到更高的特异性,使得精确的染色质概况.

结论:

  • 这种新型的微流体平台有效地缩小了CUT&Tag试验,克服了常见的挑战.
  • 拉哈马斯促进了低输入和罕见细胞样本的精确染色体分析,具有增强的特异性.
  • 这项技术有可能在各种研究应用中推进生物分析.