相关实验视频
Updated: Sep 16, 2025

08:28
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Published on: September 19, 2017
8.1K
评估G4连接体对G四重复体及其结合蛋白之间的相互作用的影响
Yuma Tanaya1, Manaho Sashida1, Hisao Masai2
1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, Koganei city, Tokyo 184-8588, Japan. tera@go.tuat.ac.jp.
概括
在生物学中,G四复合体 (G4s) 和它们的结合蛋白 (G4BPs) 是至关重要的. 新的研究表明,G4配体可以干扰G4-G4BP相互作用,即使具有高G4亲和力.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 药物发现 药物发现 药物发现
背景情况:
- G四重复体 (G4s) 是参与调节基因表达的非正规DNA结构.
- G4s与各种G4结合蛋白 (G4BPs) 相互作用,调节生物功能.
- 了解这些相互作用是开发有针对性的治疗方法的关键.
研究的目的:
- 研究G4联体对G4s和G4BPs之间的相互作用的影响.
- 开发一种定量试验,用于研究在配体的存在下G4-G4BP复合体的形成.
- 要确定G4对G4结构的高亲和力是否转化为G4-G4BP结合的调制.
主要方法:
- 开发一个三明治ELISA系统来测量G4-G4BP复合形成.
- 使用ELISA来评估各种G4对G4-G4BP相互作用的影响.
- 在不同的联结体度下对三元复合体形成的量化.
主要成果:
- G4配体显著影响G4s和G4BPs之间的结合亲和力.
- 开发的三明治ELISA系统有效量化了G4-G4BP相互作用.
- 一个G4联体对G4结构的高结合亲和力不一定会导致G4BPs增强的三元复合体形成.
结论:
- G4配体可以破坏或调节G4-G4BP复合体的形成.
- 对G4-G4BP相互作用的联结体诱导效应是复杂的,并且取决于超出简单G4结合亲和度的因素.
- 这些发现对设计针对G4的药物有意义,这些药物旨在调节蛋白质-G4相互作用.
相关概念视频
GTPases and their Regulation
8.6K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.6K
Ligand Binding and Linkage
4.9K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.9K
Activation and Inactivation of G Proteins
7.7K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
7.7K
G-protein Coupled Receptors
121.6K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
121.6K
Ligand Binding Sites
13.4K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
13.4K
GPCR Desensitization
6.5K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.5K

