相关实验视频

Updated: Sep 16, 2025

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Published on: July 9, 2014

糖酸脱酶调节膜巨细胞的恒常状态和代谢完整性

Seung Geun Song^1,2, Ein Lee¹, Jaemoon Koh²

¹Laboratory of Immune Regulation, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea.

|July 7, 2025

概括

酸脱酶复合体亚单元A (SDHA) 对于膜巨细胞 (AM) 的生存和功能至关重要. 它的缺失导致AMs中的代谢转变,细胞死亡和脂质积累,影响肺部平衡.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
代谢途径代谢途径

背景情况:

肺组织寄存的巨细胞,包括膜巨细胞 (AMs) 和间歇性巨细胞 (IMs),对于免疫防御和组织稳定至关重要.
已知AMs和IMs具有不同的功能作用,但它们的特定代谢基础仍然不清楚.
对比的RNA测序揭示了酸脱酶复合体亚单元A (SDHA) 在AMs和IMs中高度表达和差异调节.

研究的目的:

调查SDHA在调节AM代谢和功能的作用.
确定SDHA缺乏对AM群体和恒温的影响.
阐明在AMS中SDHA损失的代谢和细胞后果.

主要方法:

对比RNA测序分析以确定差异表达的基因.
基因删除SDHA以评估其在AMS中的作用.
对缺乏SDHA的巨细胞的代谢分析和转录分析.
评估细胞活力,脂质积累和内质网膜压力.

主要成果:

SDHA 删除显著减少了 AM 种群,同时节省了 IM,这表明了 AM 特定的要求.
缺乏SDHA的AMs表现出对糖解的代谢重编程.
在SDHA缺乏的AM中观察到转录变化,细胞死亡,脂质积累和内质网膜应激增加.
IMs没有显示类似的代谢或人口变化,突出显示AM特异性.

结论:

SDHA是AM新陈代谢和恒温的关键调节者.
通过SDHA保持代谢完整性对于AM功能和在肺微环境中的生存至关重要.
这些发现提供了关于组织内专门免疫细胞的代谢依赖性的见解.

关键词:

膜细胞巨细胞.恒常状态 (Homeostasis) 是一种恒常状态.代谢重编程是一种代谢重编程.线粒体中的线粒体.Succinate 脱水酶的作用.

更多相关视频

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Published on: November 15, 2024

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure

Published on: October 22, 2019

相关概念视频

Respiratory Regulation of Acid-Base Balance

Respiratory Regulation of Acid-Base Balance

Respiratory compensation is a vital physiological process that stabilizes blood plasma pH by regulating the partial pressure of carbon dioxide (PCO2), a key determinant of pH levels. Most carbon dioxide in the blood dissolves and converts into carbonic acid (H2CO3). It dissociates into hydrogen ions (H+) and bicarbonate ions (HCO3⁻). There is also an inverse relationship between PCO2 and pH.
When carbon dioxide levels increase in the blood, more H+ and HCO3⁻ are...

Sulfur Assimilation

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...

pH Regulation in Cells

pH Regulation in Cells

pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

The Electron Transport Chain

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

Physiological Control of Respiration

Physiological Control of Respiration

Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...