在急性损伤中揭示PANoptosis:一种整合性的多维方法来识别关键生物标志物.
Ning Wang1, Lina Zhang1,2, Ziyu Xu1
1Department of Nephrology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, People's Republic of China.
Journal of inflammation research
|July 7, 2025
概括
这项研究确定了五个关键基因 (EGR1,CEBPD,HSPA1A,HSPA1B,RHOB) 涉及到PANoptosis,一个编程细胞死亡过程,以及它们在急性损伤 (AKI) 中的显著升高. 这些发现为AKI诊断和治疗提供了潜在的生物标志物.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 遗传学 遗传学 是一个
背景情况:
- 编程细胞死亡和炎症是急性损伤 (AKI) 进展的核心.
- 调控细胞死亡形式的PANoptosis整合了apoptosis,pyroptosis和necroptosis,但其在AKI中的作用尚不清楚.
研究的目的:
- 为了调查PANoptosis在AKI中的作用.
- 为了确定与PANoptosis相关的AKI的潜在生物标志物.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 和批量转录基因数据被用来识别与AKI中亡,烧亡和亡相关的差异表达基因 (DEGs).
- 机器学习算法确定了关键的PANoptosis相关基因.
- 接收器操作特征 (ROC) 曲线分析评估了诊断潜力.
- 西方斑块和免疫组织化学验证了AKI动物模型中的发现.
主要成果:
- 在AKI中,PANoptosis水平显著升高,具有细胞类型特定的异质性.
- 在AKI中,五个关键的上调基因 (EGR1,CEBPD,HSPA1A,HSPA1B,RHOB) 被确定为潜在的PANoptosis生物标志物.
- 这些基因具有很高的诊断准确性 (AUC从0.920到0.981) 并在AKI模型中得到验证.
结论:
- 这项研究使用集成的转录基因数据和机器学习在AKI中确定了五个关键的PANoptosis相关基因.
- 这些基因作为AKI诊断和治疗向的潜在生物标志物.
- 这些发现为PANoptosis在AKI病变发生中的作用提供了新的见解.
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