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在炎症性肠道疾病中长非编码RNADDX11-AS1的预测值及其对肠粘膜细胞功能的影响
Tanwei Xiong1, Xiuli Wang1, Jia Li2
1Department of Gastroenterology, Chenzhou No. 1 People's Hospital, Chenzhou 423000, China.
Central-European journal of immunology
|July 7, 2025
概括
升高的长非编码RNADDX11-反感RNA1 (DDX11-AS1) 是炎症性肠病 (IBD) 的潜在生物标志物. 通过向miR-2355-5p,DDX11-AS1促进炎症和亡,提供了对IBD病变的洞察力.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 炎症性肠病 (IBD) 是一种慢性自身免疫性疾病.
- 长非编码RNAs (lncRNAs) 与IBD病变发生有关.
- 在IBD中 lncRNA DDX11-antisense RNA 1 (DDX11-AS1) 的作用需要进一步研究.
研究的目的:
- 研究DDX11-AS1在IBD中的表达和诊断价值.
- 评估DDX11-AS1对结直肠粘膜细胞的功能影响.
- 阐明DDX11-AS1在IBD中的作用背后的分子机制.
主要方法:
- 定量PCR (qPCR) 用于确定DDX11-AS1的表达.
- 接收器操作特征 (ROC) 曲线分析用于诊断价值评估.
- 在体外细胞培养模型 (FHC细胞) 使用脂多糖 (LPS) 诱导.
- 细胞计数工具-8 (CCK-8),流细胞计量和ELISA用于功能测试.
- 生物信息预测 (ENCORI) 和 luciferase 记者测试以验证目标基因相互作用.
主要成果:
- 在IBD患者中,DDX11-AS1表达显著上调.
- DDX11-AS1显示了IBD的高诊断准确性.
- 在FHC细胞中,LPS诱导的炎症和亡通过降低DDX11-AS1.1的调节来减弱.
- 证实DDX11-AS1可以直接向和反向调节miR-2355-5p.
结论:
- 上调的DDX11-AS1是一种IBD的潜在诊断生物标志物.
- DDX11-AS1通过向miR-2355-5p而加剧LPS诱导的结直肠细胞的亡和炎症.
- 这些发现为IBD病变的分子机制提供了新的见解.
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