释放对抗和生物膜的海斯素潜力:准细胞外基质,同时保持口腔细胞完整性
Luana Mendonça Dias1,2, Lina M Marin1, Ana Claudia Pavarina2
1College of Dentistry, University of Saskatchewan (USASK), 105 Wiggins Road, Saskatoon, SK S7N 5E4, Canada.
ACS omega
|July 7, 2025
概括
海斯3 (His3) 和海斯5 (His5) 蛋白质有效地降低了假牙生物膜活力和细胞外矩阵组件. 这些发现表明His3和His5对治疗假牙牙炎有前途.
科学领域:
- 生物材料科学 生物材料科学
- 口腔微生物学 口腔微生物学
- 生物化学 生物化学
背景情况:
- 假牙口腔炎影响着高达60%的假牙使用者.
- 主要由Candida albicans和Staphylococcus aureus组成的生物膜,由于其弹性细胞外基质 (ECM),导致治疗耐药性.
研究的目的:
- 为了评估四种蛋白质/的疗效:基因素3 (His3),基因素5 (His5),DR9-RR14和RR14.
- 评估它们对在烯酸树脂上培养的混合假牙生物膜的影响,重点关注可行性,ECM组件和结构.
主要方法:
- 蛋白质/被测试在先前确定的生物膜抑制度 (BIC-2).
- 分析了对生物膜活性的影响,ECM组件 (蛋白质,细胞外DNA,多糖) 和生物膜结构.
- 使用牙纤维细胞质来评估细胞毒性.
主要成果:
- 其中,His3和His5的疗效最高,分别减少了46%和41%的生物膜细胞.
- 无论是His3还是His5,都显著减少了ECM组件,并破坏了生物膜结构.
- DR9-RR14和RR14对生物膜减少有中度影响.
- 所有测试的蛋白质/被发现对牙纤维细胞是安全的.
结论:
- 立体素3 (His3) 是最有效的蛋白质/被评估抑制生物膜形成和ECM成熟.
- 希斯3显示出作为预防和治疗假牙口腔炎的治疗剂的巨大潜力.
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