氧胆酸调节肠道微生物群和胆酸代谢,以增强小猪的肠道屏障功能
Jie Chong1,2, Yongming Zhou3, Zhi Li3
1Chongqing Academy of Animal Sciences, Chongqing, China.
Frontiers in veterinary science
|July 7, 2025
概括
氧化酸 (HDCA) 影响小猪肠道微生物和肠道健康. 虽然HDCA抑制了体重增加,但便微生物群移植 (FMT) 减轻了这一点,表明微生物群对生长和肠道屏障功能的依赖性影响.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 动物科学动物科学
背景情况:
- 口服胆汁酸,如氧化胆汁酸 (HDCA),对于肠道微生物发育至关重要.
- 与小鼠相比,HDCA在小猪中表现出不同的胃肠道过境和吸收模式.
- 了解HDCA对小猪肠道微生物群和宿主新陈代谢的影响对于猪的营养至关重要.
研究的目的:
- 研究口服HDCA补充剂对小猪肠道微生物群组成和宿主代谢相互作用的影响.
- 评估HDCA对肠道屏障完整性和炎症反应的影响.
- 探索肠道微生物群在调解HDCA对小猪生长和新陈代谢的影响中的作用.
主要方法:
- 建立了四个小猪组:带有或没有HDCA的无菌小猪和接受便微生物群移植 (FMT) 的无菌小猪.
- 用16S rRNA测序分析了肠道微生物组成.
- 通过测量紧结蛋白表达和促炎性细胞因子水平来评估肠道屏障功能.
- 量化了与胆汁酸代谢相关的基因 (CYP7A1,TGR5) 的胆汁基因表达.
主要成果:
- HDCA的使用抑制了小猪的体重增加,而FMT则减轻了这种效应.
- HDCA显著改变了肠道微生物群,增加了Lactobacillus,减少了Streptococcus和Erysipelotrichaceae.
- HDCA通过调高紧密结蛋白和降低促炎细胞因子来增强肠道屏障完整性.
- 在无菌和接受FMT治疗的小猪中,HDCA上调了叶CYP7A1和TGR5基因表达.
结论:
- HDCA对小猪生长,肠道屏障功能和胆酸代谢产生微生物群依赖的影响.
- 尽管初始体重抑制,但HDCA补充可能通过TGR5激活和有益细菌丰富改善肠道屏障完整性.
- 早期的肠道微生物群在调节对小猪对HDCA等营养干预措施的反应方面发挥着关键作用.
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