在HCT中的长度蛋白质变化 116结肠癌在生长过程中的球状球体
Catherine B Whitney1, Nicole C Beller1, Brian D Fries1
1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, United States.
Journal of proteome research
|July 7, 2025
概括
球状体生长时间显著影响其生物特征. 早期的球状体 (第2天) 比长期培养更好地模拟血管瘤,这凸显了药物开发中需要精确的模型选择的需要.
科学领域:
- 生物医学工程 生物医学工程
- 蛋白质组学是指蛋白质组学.
- 癌症建模 癌症建模
背景情况:
- 美国食品和药物管理局现代化法案2.0允许先进的微生理系统,如球体,用于药物测试.
- 球体越来越多地被用作疾病模型,但生长时间经常被忽视.
- 蛋白质组学主要验证了球形实用性,没有跟踪纵向变化.
研究的目的:
- 为了研究HCT 116球体在18天的生长过程中的时间蛋白质变化.
- 评估球状体生长时间如何影响它们与无血管瘤的相似性.
- 为了确定在临床前药物开发中用于球状体的最佳生长阶段.
主要方法:
- 采用气相分离 (DIA-GPF) 蛋白质组的数据独立获取被应用.
- 在18天的时间里,每两天分析一次HCT 116球体.
- 进行了差异表达分析和基因本体学 (GO) 术语分析.
主要成果:
- 在所有球形样本中,发现了6835种蛋白质.
- 在第二天,球体与单层细胞的相似性较大,相比较于以后的时间点.
- 球状体成熟涉及DNA复制的下调和糖解的上调.
结论:
- 球状体生长时间是准确的疾病建模的关键因素.
- 早期的球状体 (第二天) 更多地代表了无血管瘤.
- 蛋白质组分析提供了关于球状时态动态和成熟过程的见解.
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