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在Helicobacter pylori感染和胃癌之间探索共享miRNA-mRNA签名网络:一项比较研究

Amal Fahma¹, Suhail Subair¹, Krishnapriya Ramakrishnan¹

¹Centre for Integrative Omics Data Science (CIODS), Yenepoya (Deemed to Be University), Mangalore, 575018, India.

Current microbiology

|July 7, 2025

概括

这项研究确定了参与Helicobacter pylori感染和胃癌 (GC) 进展的功能microRNAs (miRNAs). 这些关键的miRNA及其mRNA标为开发用于GC的基于miRNA的新型疗法提供了潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

胃癌 (GC) 是全球领先的癌症,与Helicobacter pylori (H. pylori) 感染密切相关.
微RNAs (miRNAs) 是关键的基因调节剂,与H. pylori感染和GC发育有关.
识别驱动这些病理的功能性miRNA对于治疗开发至关重要.

研究的目的:

在H. pylori感染和GC中编译和分析差异表达的miRNA.
构建miRNA-mRNA网络以识别具有反向表达模式的功能miRNA.
在H. pylori感染和GC感染中发现共享的功能miRNA及其标.

主要方法:

利用GEO数据集和文献策划来识别H. pylori感染细胞和GC组织中的人类差异表达miRNAs (hDEmiRs).
将hDEmiR目标与差异表达的mRNA (hDEmRNA) 进行比较,以建立miRNA-mRNA网络.
使用TCGA数据验证了功能miRNA-mRNA网络,并确定了共享的功能hDEmiRs.

主要成果:

在H.pylori感染中确定了24个上调和10个下调的功能性miRNA,在GC中确定了38个上调和7个下调的功能性miRNA.
发现了功能性miRNAs的共享子集,包括高调的hsa-miR-98-5p和低调的hsa-miR-204-5p,这两种条件都是共同的.
在H. pylori感染和GC. pylori感染中,确定了氧基-CoA脱酶 (HADH),ESRP2和DBT作为潜在的mRNA标,由功能性miRNA降低调节.

结论:

一组功能性miRNA及其mRNA点在H. pylori感染和GC病变发生过程中发挥关键作用.
在H. pylori感染和GC之间共享的功能性miRNAs表明疾病进展的共同途径.
这些已识别的功能性miRNAs代表了针对胃癌的向治疗策略的有希望的候选人.

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