相关实验视频
Updated: May 16, 2026
10:40
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Published on: June 5, 2020
16.7K
全架视网膜器官可视化与细胞分辨率
Marina Cunquero1, Helena Isla-Magrané2, Maria Marsal1
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology.
Journal of visualized experiments : JoVE
|July 7, 2025
概括
从干细胞中提取的人类视网膜器官,提供个性化医学见解. 新的3D成像技术可视化视网膜细胞结构,推进疾病研究和治疗策略.
科学领域:
- 生物医学工程 生物医学工程
- 发育生物学 发展生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 来自诱导多能干细胞 (iPSC) 的人类视网膜器官 (HRO) 模仿视网膜结构和功能.
- 对于研究视网膜发育,疾病和个性化医学来说,HROs非常有价值.
- 鉴定复杂的HRO是具有挑战性的,因为它们的3D架构和细胞密度.
研究的目的:
- 开发和应用先进的3D成像技术,用于全面的HRO表征.
- 为了可视化视网膜神经元在整体安装HRO中的空间组织.
- 为了提高对HRO成熟和细胞分布的理解.
主要方法:
- 结合光学清除与免疫标记,用于整体HRO可视化.
- 使用共聚焦显微镜,兼容各种数值孔径,用于全体积成像.
- 应用了一种清除方法,以保持特定区域的高分辨率成像的3D背景.
主要成果:
- 成功可视化了视网膜关键神经元的3D形态和分布 (光受体,双极细胞,质细胞).
- 展示了一种复杂的视网膜器官结构的高分辨率全体积成像方法.
- 提供了关于HROs中神经元通路的空间组织的见解.
结论:
- 光学清除与免疫标记和共聚焦显微镜相结合,对于3D HRO分析是有效的.
- 这种技术有助于研究视网膜发育和使用有机体进行疾病建模.
- 这些发现有助于更深入地了解治疗应用的视网膜器官中的细胞组织.
相关概念视频
01:56Ethics in Research
Today, scientists agree that good research is ethical in nature and is guided by a basic respect for human dignity and safety. However, this has not always been the case. Modern researchers must demonstrate that the research they perform is ethically sound.
03:20The Stanford Prison Experiment
The famous and controversial Stanford Prison Experiment, conducted by social psychologist Philip Zimbardo and his colleagues at Stanford University, demonstrated the power of social roles, social norms, and scripts.
02:04Bullying
A modern form of aggression is bullying. As you learn in your study of child development, socializing and playing with other children is beneficial for children’s psychological development. However, as you may have experienced as a child, not all play behavior has positive outcomes. Some children are aggressive and want to play roughly. Other children are selfish and do not want to share toys. One form of negative social interactions among children that has become a national concern is bullying.
01:43Sex Linked Disorders
Like autosomes, sex chromosomes contain a variety of genes necessary for normal body function. When a mutation in one of these genes results in biological deficits, the disorder is considered sex-linked.
01:26Sexually Transmitted Infections
Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...
01:28Conduct Disorder
Conduct disorder is a complex mental health diagnosis characterized by a repetitive and persistent pattern of behavior that violates societal norms, the rights of others, or age-appropriate rules. The diagnostic criteria for conduct disorder require the presence of at least three problematic behaviors within the past 12 months, with at least one occurring in the past six months. These behaviors are grouped into four categories: aggression toward people and animals; destruction of property;...