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结膜活检和直接免疫光技术用于诊断粘膜皮虫病

Tirupathi Rao¹, Sayan Basu², Swati Singh³

¹Prof. Krothapalli Ravindranath Ophthalmic Research Biorepository, LV Prasad Eye Institute.

Journal of visualized experiments : JoVE

|July 7, 2025

概括

眼膜粘膜皮炎 (MMP) 的诊断依赖于结膜活检与直接免疫光. 这种方法证实了MMP,将其与其他疾病区分开来,并指导及时治疗以预防失明.

科学领域:

眼科医生眼科眼科
免疫皮肤学免疫皮肤学

背景情况:

眼膜粘膜皮炎 (MMP) 导致慢性膜炎,可能导致失明.
早期诊断和免疫抑制疗法对于管理MMP和预防视力丧失至关重要.

研究的目的:

描述一种用于结膜活检和直接免疫光 (DIF) 染色用于MMP诊断的标准化技术.
突出影响活检阳性和解释DIF结果的因素.

主要方法:

结膜活检从两只眼睛的未涉及区域中采集,包括最小的结膜下组织.
使用最佳切割温度 (OCT) 介质进行样本转移和冷切割.
使用光结合抗体 (IgG,IgM,IgA) 和补充因子 (C3c,C4c) 进行直接免疫光染色.

主要成果:

阳性DIF在底层膜中显示绿色光,甚至在单个阳性抗体中也证实了MMP.
活检阳性受到活检特征,横向性,年龄和疾病活性的影响.
阳性活检可以区分MMP与药物诱导的膜炎和伪皮炎.

结论:

使用DIF的结膜活检是眼膜粘膜皮虫病的可靠诊断工具.
描述的技术确保了最佳的样品质量,用于准确的免疫光分析.
通过DIF准确的MMP诊断有助于适当的治疗和视觉保护.

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