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Physical Methods for Controlling Microbial Growth: Temperature

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...

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Updated: Sep 16, 2025

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

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Published on: October 1, 2016

在Ceratitis capitata中解码和工程温度敏感致死性,用于害虫控制.

Roswitha A Aumann^1,2, Georgia Gouvi^3,4, Maria-Eleni Gregoriou³

¹Department of Insect Biotechnology in Plant Protection, Institute for Insect Biotechnology, Justus-Liebig-University Gießen, Gießen 35394, Germany.

Proceedings of the National Academy of Sciences of the United States of America

|July 7, 2025

概括

研究人员在Lysyl-tRNA合成酶基因中发现了一种特定的突变,该基因对地中海果中温度敏感的致命性负责. 这一突破可能使得基因性别定性菌株的开发成为更有效的无菌昆虫技术程序.

关键词:

基因编辑CRISPR/Cas基因编辑遗传性分类菌株的遗传性分类菌株 lysine-tRNA结合酶 (Lysyl-tRNA合成酶,LysRS) 是一种酶.迷你基因救援救援这就是它所做的.

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科学领域:

遗传学是一个遗传学.
昆虫学昆虫学是一门学科.
分子生物学分子生物学

背景情况:

无菌昆虫技术 (SIT) 是一种环保的害虫控制方法,依赖于释放无菌昆虫.
基因性别定性菌株 (GSS) 对于高效的SIT至关重要,因为它只能释放雄性,特别是用于蚊子控制.
开发GSS一直是具有挑战性的,过去的温度敏感致命 (tsl) 基GSS在*Ceratitis capitata*中没有在其他害虫中复制.

研究的目的:

精确确定 *Ceratitis capitata* 中温度敏感致死性 (tsl) 现型的遗传基础.
为了验证已识别的突变在TSL表型中的作用.
评估在其他昆虫害虫中产生新型GSS的潜力.

主要方法:

对*Ceratitis capitata* lysine--tRNA酶 (LysRS) 基因进行了详细的遗传分析.
在野生类型菌株中引入特定的*LysRS*突变.
在不同温度条件下对工程菌株的表型评估.
使用随机集成的*LysRS*小基因组进行补充试验.

主要成果:

在 *C. capitata LysRS* 基因的特定突变被确定为TSL表型的原因.
将这种突变引入野生型菌株,成功地在热应激下复制了胚胎死亡率.
通过*LysRS*小基因的随机整合,TSL表型被逆转,证实了突变的致病作用.

结论:

鉴定到的 *LysRS* 突变是导致 *Ceratitis capitata* 中温度敏感的致命表型的原因.
昆虫物种中 *LysRS* 基因的高度保存表明,在各种害虫中可能会产生基于 tsl 的 GSS.
这项研究为通过改进GSS开发扩大SIT在农业和疾病预防中的应用铺平了道路.