相关实验视频
Updated: Sep 16, 2025

07:50
Establishment of a Clinic-based Biorepository
Published on: May 29, 2017
6.6K
p63和ZNF148合作调节头部和部状细胞癌
Rosalba Pecorari1, Mara Mancini1,2, Artem Smirnov1,2
1Istituto di Ricovero e Cura a Carattere Scientifico Istituto Dermopatico dell'Immacolata (IDI-IRCCS), Biochemistry Laboratory, Rome 00144, Italy.
概括
研究人员确定了一条涉及p63和ZNF148的新分子途径,通过过度表达环素D1.1,驱动头部和部状细胞癌 (HNSCC) 的进展. 这一癌症特异性轴为先进的HNSCC提供了潜在的新治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 头部和部状细胞癌 (HNSCC) 是一种具有攻击性的恶性瘤,对于晚期的治疗具有挑战性.
- 瘤基因p63是一种频繁放大的转录因子,在HNSCC的发病过程中起着至关重要的作用.
- 了解p63的相互作用是开发针对HNSCC的向治疗的关键.
研究的目的:
- 在HNSCC中使用蛋白质组方法识别p63瘤基因的结合伙伴.
- 阐明p63对HNSCC进展作出贡献的分子机制.
- 发现高级HNSCC的潜在治疗点.
主要方法:
- 蛋白质组分析以确定p63结合伙伴.
- 共同免疫沉和西部涂抹以确认蛋白质相互作用.
- 基因组占用分析 (例如,ChIP-seq) 用于绘制结合部位的地图.
- RNA测序和定量PCR用于评估基因和eRNA表达水平.
主要成果:
- 确定ZNF148作为一个在HNSCC细胞中同表达的p63结合伙伴.
- 在CCND1基因上游被p63和ZNF148.8所占用的功能增强剂衍生RNA (eRNA) 发现.
- 证明p63和ZNF148控制eRNA转录,导致CCND1过度表达并促进瘤细胞增殖.
- 证实这个轴是针对癌细胞的特异性,在正常表皮上是不活跃的.
- 在p63,ZNF148,eRNA和先进的HNSCC阶段和转移之间发现了表达水平的正相关性.
结论:
- 一个涉及p63,ZNF148和CCND1调节的eRNA的新型分子途径驱动HNSCC的进展.
- 这一途径是癌症特异性的,突出其作为选择性治疗点的潜力.
- 针对这一轴可以为高级HNSCC和转移性疾病提供新的治疗策略.
相关概念视频
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Negative Regulator Molecules
36.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.1K
DNA Damage can Stall the Cell Cycle
9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
Interactions Between Signaling Pathways
6.5K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.5K
Covalently Linked Protein Regulators
7.3K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
7.3K
Molecular Factors Affecting Cell Division
3.3K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.3K

