研究来自Hippophae rhamnoides的水性叶子同质物的冷特性
Vitalii Mutsenko1, Evgenii Rubalskii2, Elias Anastassopoulos3
1Institute for Multiphase Processes, Leibniz University Hannover, Garbsen, Germany; Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, Hannover, Germany.
Cryobiology
|July 7, 2025
概括
河 (Hippophae rhamnoides) 的叶子含有来自植物和细菌来源的冰核化剂 (INA),有助于适应寒冷. 这些发现对环境和冷生物应用有价值.
科学领域:
- 低温生物学 低温生物学
- 植物科学 植物科学
- 微生物学 微生物学
背景情况:
- 河 (Hippophae rhamnoides) 具有显著的耐寒性,可以在低至-40°C的温度下生存.
- 众所周知,该植物具有内在的冰核化剂 (INA),并含有可能导致冰形成的细菌.
研究的目的:
- 为了研究Hippophae rhamnoides粗叶同质物 (CLHs) 与冷保护剂 (CPAs) 的结模式.
- 确定H. rhamnoides中冰核形成的来源,区分植物来源和微生物贡献.
主要方法:
- 红外热学,冷显微镜和差分扫描热量计 (DSC) 用于分析结模式.
- 微生物学研究,包括有氧培养和16S rRNA测序,在CLH上进行,以确定细菌冰核子.
主要成果:
- 将CLH添加到分糖酸中,增强了结晶,并减少了超冷和冷时间,类似于SnomaxTM.
- 过和热处理的CLH表明存在来自植物和细菌来源的可溶性冰核子.
- 鉴定了三种来自Erwiniaceae家族的细菌分离物,包括Pantoea agglomerans和Duffyella gerundensis,已知的冰核物种.
结论:
- Hippophae rhamnoides的叶子含有来自植物的内在和外源的细菌冰核化剂.
- 这些发现有助于了解植物适应寒冷,并具有潜在的冷生物和环境应用.
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