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系统工程 Komagataella phaffii用于生产母蛋ovomucoid的生产

Hyungdong Kim¹, Kyoung Chan Jin¹, Sun-Ki Kim¹

¹Department of Food Science and Biotechnology, Chung-Ang University, Anseong, Gyeonggi 17546, Republic of Korea; GreenTech-based Food Safety Research Group, BK21 Four, Chung-Ang University, Anseong, Gyeonggi 17546, Republic of Korea.

International journal of biological macromolecules

|July 7, 2025

概括

这项研究优化了使用Komagataella phaffii酵母的ovomucoid (OM) 生产. 研究人员增强了OM分泌,为这种生物活性蛋白蛋白获得了929mg/L的高产.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
蛋白质生产蛋白质生产

背景情况:

卵 (OM) 是卵白中的关键生物活性蛋白,有助于其独特的特性.
对于各种应用来说,高效和环保的OM生产是理想的.
科马格塔 (Komagataella phaffii) 是一个适合分泌蛋白质生产的宿主.

研究的目的:

开发一种优化的环保技术,用于使用Komagataella phaffii.使用的卵子 (OM) 生产.
确定最佳的信号和促进剂,以增强OM分泌.
为了减轻内质网膜 (ER) 的压力,并最大限度地提高细胞外OM产量.

主要方法:

在K. phaffii中选信号和促进剂以检测OM分泌.
通过协同表达HAC1和PDI1来设计分泌途径,以减少ER压力.
优化高细胞密度培养以获得最大的细胞外OM产量.

主要成果:

EPX1信号和GCW14促进剂的组合在测试结构中产生了最高的OM度 (23 mg/L).
HAC1和PDI1的同时表达显著提高了OM分泌效率.
在高细胞密度培养下,最大化的细胞外OM产量达到929 mg/L.

结论:

一个基于K. phaffii的优化分泌系统已经成功地开发出来,用于产卵子.
设计未折叠的蛋白质反应途径对于有效的蛋白质分泌至关重要.
开发的技术提供了一种可扩展和环保的方法来生产生物活性卵子.

关键词:

高细胞密度培养方式科玛格塔贝拉 (Komagataella phaffii) 是一个有趣的植物.卵子状的卵子

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