PsMYB4与PsEGL3的相互作用抑制了树黄花中的安托西亚尼生物合成

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Updated: Sep 16, 2025

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Xiaoning Luo¹, Sijie Huang¹, Fangjun Jiang¹

¹College of Landscape Architecture, Central South University of Forestry and Technology, Hunan, Changsha, China.

Frontiers in plant science

|July 8, 2025

概括

两种转录因子,PsMYB4和PsEGL3,被确定为树黄色花的形成的关键调节者. 它们相互作用抑制安托西亚尼合成,重定向代谢流向黄醇.

科学领域:

植物分子生物学植物分子生物学
生物化学生物化学
遗传学遗传学是一种遗传学.

背景情况:

树 (Paeonia suffruticosa) 由于其装饰价值而受到重视,特别是黄色品种.
在树中黄色花色素的分子基础在很大程度上是未知的.
了解这种机制对于育种和园艺进步至关重要.

研究的目的:

阐明控制树黄花形成的分子机制.
为了确定关键的基因和转录因子参与色素生物合成途径.
研究已识别的转录因子的相互作用和功能.

主要方法:

在树和烟草中进行基因表达分析 (过度表达和沉默).
对已识别的转录因子进行遗传学分析.
酵母两杂交 (Y2H) 和双分子光补充 (BiFC) 测定蛋白质相互作用.
双露西法酶记者测定用于评估促销者活动.

主要成果:

两种转录因子,PsMYB4 (R2R3-MYB抑制剂) 和PsEGL3 (bHLH家族),在黄色品种中表达高.
过度表达PsMYB4和PsEGL3抑制了烟草中的安东氨酸合成,而PsMYB4显示出更强的效果.
在树花中抑制PsMYB4和PsEGL3可以调节上升的安托素生物合成基因.
PsMYB4和PsEGL3在物理上相互作用,并协同抑制关键安托素生物合成基因 (PsCHS,PsCHI,PsDFR) 的促进者.

结论:

PsMYB4 和 PsEGL3 作为树树中安托素生物合成的抑制剂.
它们的相互作用将新陈代谢流从酸转向黄醇,从而产生黄色的花朵颜色.
这项研究为树的花色素的遗传调节提供了新的见解.

关键词:

在 PsEGL3 中,我们可以使用 PsEGL3.这就是PsMYB4的原因.安托氨酸生物合成黄酸路径的路径负面监管是一种消极的监管.树木梨花梨花黄色的花朵黄色的花朵

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