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Updated: Sep 16, 2025

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

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在慢性感染期间在ART启动后,各种HIVDNA区域和HIV转录的差异性下降

Julie Janssens¹, Cordelia Isbell¹, Sun Jin Kim¹

¹Department of Medicine, University of California, San Francisco (UCSF), San Francisco, California, USA.

Journal of virology

|July 8, 2025

概括

抗逆转录病毒疗法 (ART) 减少了大多数HIV转录,但仍存在一些不完整或有缺陷的病毒RNA,可能会导致艾滋病毒感染者的炎症. 需要新的疗法来清除剩余的HIV储存库和转录.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

在抗逆转录病毒疗法 (ART) 期间,转录HIV的细胞会持续存在,在治疗停止后可能导致炎症和病毒反弹.
开始ART的时间和持续时间对不同进程性和突变的HIV转录的清除的影响仍然不清楚.

研究的目的:

研究ART开始时间和持续时间如何影响不同HIV转录及其相应DNA的清除.
了解长期ART中外围CD4+T细胞中HIVRNA和DNA水平的动态.

主要方法:

从10个人的CD4+T细胞中对HIVDNA和各种HIV转录的纵向分析.
采样发生在未经治疗的慢性艾滋病毒感染期间,以及在抑制性ART (超过7年) 的最多四个时间点.
量化启动,延长,完成,多重拼接,完整和缺陷的HIVRNA相对于HIVDNA.

主要成果:

ART减少了大多数HIV转录;多重拼接和完整的HIVRNA分别比启动和5'缺陷RNA下降得更快.
虽然大多数转录在ART治疗1年后稳定,但多重拼接RNA持续下降长达3年,5'缺陷DNA长达5年.
完整的HIVRNA变得无法检测,但不完整/有缺陷的转录达到了平衡,表明ART和免疫系统的局限性.

结论:

ART不同影响各种HIV转录的清除,一些不完整或有缺陷的形式长期存在.
持久的病毒产物,即使在低水平,也可能导致正在进行的免疫激活和炎症治疗的个体.
需要新的治疗策略来准持久的HIV储存和转录,以实现完全的病毒根除和减少免疫激活.

关键词:

艺术艺术艺术艺术它们是DNA DNA DNA DNA.艾滋病病毒艾滋病病毒艾滋病病毒艾滋病病毒-1 艾滋病病毒-1 这是一个RNARNARNARNARNA.转录转录是一种转录.

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