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Updated: Sep 16, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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在使用单链DNA辅助双链DNA尼克的重复序列上进行PCR免费的位点定向突变发生,由DNA酶进行

Mingkuan Lyu1, Linggen Kong2,3, Xiangli Shao1

  • 1Department of Chemistry, The University of Texas at Austin, Austin, TX, 78712, USA.

Angewandte Chemie (International ed. in English)
|July 8, 2025
PubMed
概括

一种新的无PCR方法使用DNA酶在具有挑战性的重复性DNA序列上进行位点定向突变发生 (SDM). 这种DNA分裂DNA酶系统 (DANDA) 为基因工程提供了一种具有成本效益和高效的替代方案.

关键词:
在DNA裂变的过程中,这就是DNAzyme DNA酶.有氧核酸类的部分.局部导向的突变发生.

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