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Updated: Sep 16, 2025

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
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光遗传学和化学遗传工具用于快速重新定位维门中间丝

Milena Pasolli1, Joyce C M Meiring1, James P Conboy2

  • 1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands.

The Journal of cell biology
|July 8, 2025
PubMed
概括

研究人员开发了新的工具,以快速操纵中间丝 (IF),特别是维蒙丁. 这使得研究立即效应成为可能,揭示了维门丁的存在.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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