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长期热应激对Hyphantria cunea (Lepidoptera: Erebidae) 种群动态和HSP70表达的影响

Shiyue Zhao^1,2, Fei Teng^1,2, Guifang Zhong^1,2

¹Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

Journal of economic entomology

|July 8, 2025

概括

昆虫面临热应激,极端温度降低了它们的生存率. 热冲击蛋白70 (HSP70) 基因表达在高温下增加,有助于耐热性,并影响害虫种群动态.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
生态生态学生态生态学

背景情况:

虫 (Hyphantria cunea) 是一个重要的隔离害虫,正在扩大其在中国的范围.
越来越多的热应激对昆虫种群构成了挑战.
热冲击蛋白70 (HSP70) 对于昆虫的耐热性至关重要,但它在恒温下对H. cunea的作用还未得到充分研究.

研究的目的:

研究热应激对Hyphantria cunea生存,发育和繁殖的影响.
在H. cunea. 中克隆和表征HSP70基因.
了解HSP70在H. cunea.中介热耐性的作用.

主要方法:

在不同发育阶段,H. cunea幼虫暴露于极端热处理 (EHT) 和热处理 (HT).
来自H. cunea. 的四个HSP70基因的克隆和表征.
对HcHSP70s的遗传学分析.
在热应激下对HcHSP70基因表达的定量分析.

主要成果:

EHT大大降低了H. cunea幼虫的存活率,并阻止了生命周期的完成.
在晚期幼虫阶段的HT缩短了发育,降低了生育能力,降低了健康状况.
鉴定了四个HcHSP70基因,显示出高保护性和与Noctuidae昆虫的遗传学相似性.
在热应激下,HcHSP70的转录显著上调,有日间波动.

结论:

H. cunea对中等热量有耐受性,但在繁殖前的热应激下,其生育能力降低.
提高HSP70表达的调节有助于提高H. cunea. 的耐热性.
这项研究为预测热压地区的H. cunea种群提供了洞察力.

关键词:

年龄阶段双性生活表.基因表达的基因表达方式分子陪伴者是一个分子陪伴者.人口健身的人口健身.热承受能力热承受能力

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