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Updated: Sep 16, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

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化学交联对蛋白质和蛋白质组件结构的影响

Tamar Tayri-Wilk1, Nir Kalisman2,3, Uri Raviv1,2

  • 1Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, 9190401 Jerusalem, Israel.

Analytical chemistry
|July 8, 2025
PubMed
概括

甲 (FA) 交叉链接显著改变蛋白质结构,不同于 bis (sulfosuccinimidyl) 亚基酸盐 (BS3). 小角度X射线散射 (SAXS) 证实FA诱导聚合,突出BS3作为结构生物学研究的首选.

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Ligand Binding and Linkage

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The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
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