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使用双酶氧化还原系统进行酶开采和D-塔加合成

Yin Fang¹, Jiangbo Li², Na Li¹

¹College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China.

Biochemical and biophysical research communications

|July 8, 2025

概括

研究人员开发了一种高效的D-塔加生产方法,使用大肠杆菌中的双酶系统. 这促进了低热量甜味剂D-塔加的合成,为治疗糖尿病和肥胖提供了一个有前途的替代方案.

科学领域:

生物技术是生物技术.
酵素工程是什么意思酵素工程
代谢工程是代谢工程.

背景情况:

全球糖尿病和肥胖症的增加需要更健康的甜味剂替代品.
低热量甜味剂D-塔加具有治疗潜力,但面临生产挑战.
目前使用L-阿拉比诺酸异构酶或氧化还原酶的D-塔加合成方法在转换速率和净化方面存在局限性.

研究的目的:

开发一种高效的D-塔加生产方法,使用一种新的双酶氧化还原系统.
为了克服热力学平衡和低酶活性在以前的D-塔加合成路径中的局限性.
设计一个大肠杆菌细胞工厂,以优化D-塔加的生物合成.

主要方法:

酶查发现了来自Meyerozyma guilliermondii (MgXR) 的高活性氧化糖还原酶和来自Rhizobium meliloti (RmGDH) 的银醇脱酶.
在大肠杆菌中MgXR和RmGDH的同时表达BL21(DE3) 用于D-银糖转化.
实施NADPH再生系统以提高反应效率.
使用乳糖作为基质的工程化大肠杆菌MgRm-trcf菌株的料批发发酵.

主要成果:

MgXR的活性为42.2 ± 1.1 U/mg,而RmGDH的活性为10.2 ± 0.8 U/mg.
同表达产生了从D-银糖中初始的D-塔加位15.48 g/L.
添加NADPH再生系统增加了D-塔加标位,达到21.3g/L.
料批发发酵产生了创纪录的34.7g/L D-塔加,生产率为0.482g/L·h.

结论:

工程化的大肠杆菌细胞工厂具有双酶氧化还原系统,为D-塔加合成提供了高效的途径.
这项研究表明,D-塔加的生物技术生产取得了重大进展,超过了以前的产量.
开发的方法提供了一种可持续和可扩展的方法来生产D-塔加,这是一种有价值的甜味剂,适用于健康意识的市场.

关键词:

共同表达是一种共同表达.D - 塔加托糖 D - 塔加托糖酶查酶查酶查酶查稀有糖是一种罕见的糖.氧化还原剂的使用方法

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