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来自Camptotheca acuminate的内性真菌中坎普托西因生物合成的表征

Tong Zhang¹, Zhiqiang Tao¹, Siyi Zhou¹

¹Shanghai Engineering Research Center of Plant Germplasm Resources, Shanghai Collaborative Innovation Center of Plant Germplasm Resources Development, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

Plant physiology and biochemistry : PPB

|July 8, 2025

概括

一种内菌,Aspergillus tennesseensis,被发现可以产生抗癌化合物坎普托素 (CPT). 这种真菌显示出作为替代CPT来源的潜力,甲醇显著提高了其产量.

科学领域:

自然产品化学自然产品化学
菌类学菌类学是指菌类学.
生物技术是生物技术.

背景情况:

坎普托西因 (CPT) 是一种重要的抗癌类化合物,但从植物中提取它限制了供应.
内生真菌为制药化合物提供了一个有希望的替代来源.

研究的目的:

隔离和识别一种能够产生坎普托西因 (CPT) 的内菌.
为了研究生物合成途径和产量增强中发现的真菌中CPT.
探索真菌内植物作为CPT生产可持续来源的潜力.

主要方法:

从Camptotheca acuminata种子中分离和识别内菌.
孤立的真菌 (Aspergillus tennesseensis) 的形态和分子特征.
使用高性能液体染色学 (HPLC) 和LC-MS. 量化CPT.
使用UPLC-QTOF-MS.识别CPT路径中间体.
评估生物转化和生产增强策略 (例如,甲醇处理).

主要成果:

一种内菌,Aspergillus tennesseensis,被确定为一个CPT生产者.
这种真菌似乎具有双重的CPT生物合成途径,由已识别的中间体证明.
A. tennesseensis 首选产生10-基坎普托西因.
甲醇处理显著增加了体 (9倍) 和培养基 (14.5倍) 的CPT产量.

结论:

阿斯珀吉勒斯 (Aspergillus tennesseensis) 是一种可行的真菌衍生的坎普托西因的来源.
该研究阐明了真菌CPT生物合成的关键方面,并确定了增强生产的强有力的诱因.
这一发现突显了A. tennesseensis作为制药CPT制造可持续替代品的潜力.

关键词:

坎普托特卡明酸盐是什么意思在Camptothecin的营地里.这是内性真菌.

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