小颗粒,大问题:聚乙烯纳米颗粒会诱导DNA损伤,氧化应激,迁移和菌原性途径,主要在非恶性肺细胞中
Büsra Ernhofer1, Andreas Spittler2, Franziska Ferk3
1Department of Thoracic Surgery, Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria.
Journal of hazardous materials
|July 8, 2025
概括
聚乙烯纳米塑料 (PS-NP) 可以改变正常的肺细胞,增加迁移和生存途径. 这些效应在肺细胞和有机体中观察到,这表明环境污染物对肺部健康的潜在风险.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 聚烯微型和纳米塑料 (PS-MNPs) 是新兴的环境污染物.
- 它们对人类健康,特别是肺组织的潜在影响需要研究.
研究的目的:
- 研究不同大小的PS-MNP对肺癌和正常肺细胞的细胞毒性作用.
- 评估PS-NP对细胞行为,DNA损伤,氧化应激和肺细胞和有机体信号通路的影响.
主要方法:
- 暴露A549,H460,DMS53,H372和BEAS-2B肺细胞,以及肺器官,到0.25微米和1微米的PS-MNP.
- 进行了细胞活力,增殖,细胞内动力学,迁移,细胞循环停止,DNA损伤,氧化应激和信号通路激活 (AKT,ERK) 测试.
主要成果:
- 在较低度下,PS-MNP不会影响细胞活力或细胞增殖.
- 与恶性肺细胞相比,非恶性BEAS-2B肺细胞显示出更高的PS-NP循环和更高的灵敏度.
- 在BEAS-2B细胞中,PS-NP诱导了增加的迁移,S相停止,DNA损伤和氧化应激.
- 在BEAS-2B细胞中,基因切除修复能力降低,AKT/ERK酸化增加.
- 这些效应在肺器官中得到了验证.
结论:
- PS-NPs并没有显著改变肺癌细胞的恶性行为.
- 通过诱导生存途径,迁移和改变应激反应,PS-NPs可能在正常肺细胞中促进恶性特征.
- 这些发现突显了PS-NP对正常肺细胞和恶性肺细胞的影响差异,并表明了对肺部健康的潜在风险.
更多相关视频
03:32A Pleural Effusion Model in Rats by Intratracheal Instillation of Polyacrylate/Nanosilica
Published on: April 12, 2019
8.0K
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
8.8K
相关概念视频
DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Spontaneous and Induced Mutations
Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
Other Unique Bacteria
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic and are commonly found near the...
Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
Atypical Pneumonia
Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...
