植物中SWEET糖载体的进化轨迹和功能多样性
Xinyang Li1, Zhongying Ren1,2, Zhiqiang Zhang1
1State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
The Plant journal : for cell and molecular biology
|July 8, 2025
概括
甜蛋白对于植物糖的运输和进化至关重要. 这项研究揭示了它们的起源在叶绿植物中,并将其多样化为负责运输不同糖的分类,这对植物的适应和发育至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子进化分子进化
- 生物化学 生化学
背景情况:
- 糖蛋白促进植物中的糖运输,影响生长,发育和应激反应.
- 了解SWEET蛋白质的演变是理解植物适应和碳水化合物代谢的关键.
研究的目的:
- 研究SWEET蛋白质在各种植物系的进化史和功能多样化.
- 阐明糖运输的结构基础和SWEET蛋白质中的基质特异性.
主要方法:
- 来自59种植物的1246种SWEET蛋白的遗传学分析.
- 保存和可变区域的序列和结构分析.
- 功能性测试以确认运输活动和基质特异性.
主要成果:
- 甜蛋白起源于叶绿素,并多样化成四个分类 (I-IV) 具有不同的糖运输功能.
- 早期的SWEETs (clade II) 缺乏运输活动;clade I SWEETs获得的葡萄糖/果糖运输;clade IV SWEETs运输果糖;clade III SWEETs运输砂糖.
- 保存的跨膜区域对于运输至关重要,而N/C终端区域影响基质的特异性.
结论:
- 这项研究为SWEET蛋白提供了一个全面的进化框架,强调它们在植物适应中的作用.
- 这些发现为糖运输的分子机制及其在植物中的进化意义提供了洞察力.
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