Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.7K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same author

Cryo-electron microscopy fuels the architectural characterization of cellular metabolism.

FEBS letters·2026
Same author

Higher-order structural organization of mitochondrial metabolism.

The Journal of biological chemistry·2026
Same author

Direct evidence of acid-driven protein desolvation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Cryo-EM structure of photosystem II supercomplex from a green microalga with extreme phototolerance.

Nature communications·2026
Same author

Mechanism of SHP2 activation by bis-Tyr-phosphorylated Gab1.

Structure (London, England : 1993)·2025
Same journal

Large-scale discovery platform enables identification of peptides targeting drug-resistant candidiasis.

Cell reports methods·2026
Same journal

A computational method to design broad-spectrum T cell-inducing vaccines applied to Betacoronaviruses.

Cell reports methods·2026
Same journal

MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum.

Cell reports methods·2026
Same journal

Induced pluripotent stem cell-derived macrophages enable broad modeling of human inflammasome signaling.

Cell reports methods·2026
Same journal

Rapid discovery of cell-surface glycosylation regulators using a lectin-based magnetic CRISPR screen.

Cell reports methods·2026
Same journal

A real-time FRET ubiquitin transfer assay for quantitative characterization of ternary complexes in targeted protein degradation.

Cell reports methods·2026
查看所有相关文章

相关实验视频

Updated: Sep 16, 2025

Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

3.9K

自主监督学习用于冷电磁显微镜中的可概括粒子采集.

Andreas Zamanos1, Panagiotis Koromilas2, Giorgos Bouritsas1

  • 1Department of Informatics and Telecommunications, National and Kapodistrian University of Athens, 16122 Athens, Greece; Archimedes Unit, Athena Research Center, 15125 Athens, Greece.

Cell reports methods
|July 8, 2025
PubMed
概括
此摘要是机器生成的。

低温电子显微镜蒙面自编码器 (cryo-EMMAE) 是一种新的自主监督方法,用于分析低温EM数据. 它有效地识别粒子并改进3D重建,优于监督方法.

关键词:
CP:计算生物学 计算机生物学CP:成像成像的使用.应用机器学习 应用机器学习细胞同质化物 细胞同质化物低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.蒙面的自动编码器微摄影机的使用情况.原生细胞提取物原生细胞提取物颗粒采集 颗粒采集蛋白质复合体 蛋白质复合体蛋白质是一种蛋白质.自主监督学习学习

更多相关视频

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.9K
Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency
06:41

Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Published on: May 10, 2024

1.9K

相关实验视频

Last Updated: Sep 16, 2025

Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

3.9K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.9K
Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency
06:41

Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Published on: May 10, 2024

1.9K

科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 计算生物学 计算生物学

背景情况:

  • 低温电子显微镜 (cryo-EM) 数据的手动注释是劳动密集型和耗时的.
  • 开发用于粒子采集的自动化方法对于高效的冷EM数据分析至关重要.
  • 现有的方法通常需要大量的注释数据集来训练监督模型.

研究的目的:

  • 引入冷电子显微镜蒙面自编码器 (cryo-EMMAE),一种用于冷电子显微镜数据分析的新型自主监督学习方法.
  • 为了证明冷-EMMAE在粒子采集和3D重建中的有效性.
  • 为了减少对冷-EM工作流程中手动注释的依赖.

主要方法:

  • 实施了面具自编码器 (MAE) 架构,用于对冷EM图像进行自我监督的学习.
  • 利用MAE的潜伏表示空间来聚类粒子像素.
  • 在不同的EMPIAR数据集上评估了冷-EMMAE性能,用于粒子采集和3D重建.

主要成果:

  • 与最先进的监督方法相比,cryo-EMMAE实现了优越的概括能力.
  • 该方法在不同培训数据集中显示出一致的性能,并且具有数据效率,只需五个微图就可以融合.
  • 在单粒子数据集和细胞提取物中观察到优异的3D重建性能.

结论:

  • 自主监督学习,以cryo-EMMAE为例,为cryo-EM图像分析提供了一个强大的替代方案.
  • cryo-EMMAE提高了结构生物学研究的效率并降低了成本.
  • 该方法显示了推进自动化冷EM数据处理的巨大潜力.