相关实验视频

Updated: Sep 16, 2025

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

Published on: March 17, 2023

小蛇头 (Channa asiatica) 的染色体水平参考基因组组合

Haiyang Liu^1,2, Jiafeng Liu^1,3, Tongxin Cui^1,4

¹Key Laboratory of Tropical and Subtropical Fishery Resources Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.

Scientific data

|July 8, 2025

概括

研究人员生成了小蛇头 (Channa asiatica) 的高质量染色体级基因组,这是一个重要的水产养殖物种. 这种基因组资源将促进繁殖计划和进化研究.

科学领域:

基因组学就是基因组学.
水产养殖是水产养殖的一种方式.
进化生物学进化生物学

背景情况:

小蛇头 (Channa asiatica) 在水产养殖和装饰品贸易中具有经济意义.
有限的基因组数据阻碍了这一物种的遗传研究和育种工作.

研究的目的:

为 Channa asiatica 构建一个高质量的染色体级基因组组件.
为未来的研究和育种提供基础的基因组资源.

主要方法:

使用了PacBio HiFi长读序列,Illumina短读序列和Hi-C技术.
组装了一个659.44Mb的基因组,其中98.18%的基因被在23个染色体上.
标注了26603个具有高度完整性的蛋白质编码基因 (98.93%的BUSCO得分).

主要成果:

为亚洲香生成了染色体级的基因组组.
实现了高位 (23.92 Mb) 和脚手架 (29.61 Mb) N50 值.
鉴定了26,603个蛋白质编码基因,并表征了重复元素 (27.72%).

结论:

高准确性基因组组装是Channa asiatica的宝贵资源.
促进了分子育种,比较基因组学和进化研究的进展.
支持对经济重要鱼类物种的未来研究.

更多相关视频

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

Published on: June 28, 2012

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...