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相关概念视频

RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K

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相关实验视频

Updated: Sep 16, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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纳米3P-seq:在单分子分辨率下绘制编码和非编码的转录组图.

Oguzhan Begik1, Leszek P Pryszcz1, Adnan Muhammad Niazi2

  • 1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.

Nature protocols
|July 8, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了纳米孔3'终端捕获测序 (Nano3P-seq) 用于分析RNA多A尾部动态. 该方法准确量化了RNA丰富度,尾巴长度和各种RNA类型的组成.

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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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3' End Sequencing Library Preparation with A-seq2
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3' End Sequencing Library Preparation with A-seq2

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相关实验视频

Last Updated: Sep 16, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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3' End Sequencing Library Preparation with A-seq2
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3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • RNA多化对RNA处理至关重要,影响稳定性,翻译和衰变.
  • 多甲尾长是mRNA功能和调节的一个关键决定因素.
  • 目前用于分析多A尾的方法在范围或分辨率上可能受到限制.

研究的目的:

  • 介绍纳米孔3'终端捕获测序 (Nano3P-seq) 的详细协议.
  • 引入PolyTailor软件,用于分析多的尾巴长度和组成.
  • 为了使单分子分辨率分析的RNA尾巴动态跨各种RNA类型.

主要方法:

  • 使用纳米孔测序开发和验证Nano3P-seq协议.
  • 使用基于模板切换的方法从3'端对cDNA进行测序.
  • 使用PolyTailor软件进行数据分析和预测尾巴特征.

主要成果:

  • 纳米3P-seq准确地估计了转录的丰度,多个A尾的长度和组成.
  • 该方法成功分析了编码和非编码RNA,包括mRNA,snoRNA和rRNA.
  • 该协议与R10.4流细胞兼容,可以在一天内完成.

结论:

  • 纳米3P-seq为研究RNA尾部动态提供了一个全面和可重复的方法.
  • 该协议可适应各种RNA样品准备方法.
  • 这种技术增强了对转录组范围内的尾巴异质性的研究.