在抗生素暴露下改变了冷震蛋白的基因表达
Evieann Cardoza1, Darsh Vira1, Advait Rao1
1Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS University, Vile Parle (West), Mumbai, India.
The Journal of antibiotics
|July 8, 2025
概括
抗生素改变了大肠杆菌中冷震蛋白 (Csps) 的表达. 不同的抗生素类别诱导不同的Csps,揭示Csps.
科学领域:
- 微生物学和分子生物学
- 细菌应激反应机制 细菌应激反应机制
背景情况:
- 蛋白质合成对环境压力因素敏感,包括温度极端和转化抑制剂.
- 寒冷冲击蛋白 (Csps) 对于细菌适应寒冷至关重要,但它们在抗生素压力下的作用在很大程度上没有被描述.
- 大肠杆菌具有九种不同的Csp基因,其在各种压力下协调或差异性表达尚未完全理解.
研究的目的:
- 研究大肠杆菌中所有九种冷震蛋白 (Csps) 的表达模式,当暴露于五种不同抗生素的次致命度时.
- 为了确定抗生素诱导的压力是否会触发Csps和潜在的热冲击蛋白 (Hsps) 的表达.
- 阐明抗生素类和特定的Csp组诱导之间的关系.
主要方法:
- 量化聚合酶链反应 (qPCR) 用于分析所有9个Csp基因的表达水平.
- 细菌生存测定在一系列亚致死抗生素度的范围内进行.
- 来自不同类别的抗生素 (氨基醇,四环素,甘胺,甘胺,安培) 用于向不同的细胞过程.
主要成果:
- 大肠杆菌中九个Csps的表达模式因不同抗生素的反应而有显著差异.
- C组抗生素 (氨基醇,四环素) 调高了冷诱导和无特征的Csp组.
- H-组 (干amycin) 和其他抗生素 (gentamicin,ampicillin) 诱导了Csps和热冲击蛋白 (Hsps).
结论:
- 抗生素压力会诱导大肠杆菌中寒冷冲击蛋白的差异表达.
- 这些发现表明,Csps在超越极端温度的细菌应激耐受性中起作用.
- 这项研究为Csp表达的触发因素及其对细菌病原和毒性的更广泛影响提供了新的见解.
更多相关视频
09:26Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
3.3K
07:59Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Published on: June 29, 2021
3.7K
相关概念视频
Other Stress Responses in Bacteria
70
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
70
Stringent Response in E. coli
60
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
60
Coordination of Gene Expression Processes in Bacteria
160
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
160
Development of Antibiotic Resistance
219
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
219
Bacterial Protein Maturation
97
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
97
Translational Regulation
106
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
106
