重复性肠球菌病的临床和基因组特征:多中心前性队列研究 (VENOUS)
Shelby R Simar1, Truc T Tran2,3, Kirsten B Rydell2,3
1Center for Infectious Diseases, UTHealth-Houston School of Public Health, Houston, TX, USA.
在复发性肠球菌血流感染中观察到基因组多样性. 虽然Enterococcus faecium不是菌株特异性的,但它显示了对持久性感染的遗传预适应,这表明差异性基因表达起着作用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 复发性肠球菌血流感染 (EBSI) 具有重大风险.
- 使用了"抗万科明素肠球菌细菌病结局研究" (VENOUS) 队列 (2016-2022年).
- 进行了反抗性EBSI的临床和基因组表征.
研究的目的:
- 调查反抗性EBSI的临床和基因组特征.
- 为了比较反抗者和非反抗者EBSI病例之间的基因组特征.
- 为了确定可能导致持续性肠球菌病的潜在适应性.
主要方法:
- 被定义为持续性细菌血症,即在主动治疗第四天缺乏清除,并且在清除后再培养后复发.
- 在匹配的非反复性细菌病患者中使用了2:1的对照:病例比.
- 进行了短读和长读全基因组测序和分离物的混合组装.
主要成果:
- 从41名患者中确定了46例反抗性感染.
- 患有持续性细菌病的患者在ICU入院率较高.
- 持续性感染中的Enterococcus faecalis菌株显示碳水化合物利用基因增加;在E. faecium和E. faecalis之间注意到了特定物种的适应性.
结论:
- 基因组多样性,而不是菌株特定的特征,特征是反抗性的EBSI分离物.
- 菌可能具有对持久性感染的遗传预适应.
- 不同的基因表达和结构变异可能有助于肠球菌在细菌病期间的适应和持久性.
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