在缺乏下,通过细胞外聚合物物质促进真菌生物活性
Shang Pan1,2, Yizhou Jiang2, Shihui Yan2
1College of Agro-Grassland Sciences, Nanjing Agricultural University, Nanjing, China.
Environmental microbiology
|July 9, 2025
概括
细胞外聚合物物质 (EPS) 作为微生物的营养来源. 这项研究表明,EPS增强了Aspergillus niger的生长,并改变了其酸溶解策略,在存在EPS和无机酸时,有利于酸酸酶而不是氧酸.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 环境科学 环境科学
背景情况:
- 细胞外聚合物物质 (EPS) 是微生物的关键营养来源.
- 了解微生物对营养可用性的反应对于生态和生物技术应用至关重要.
- 黑色是一种关键的真菌,参与营养循环和工业过程.
研究的目的:
- 研究细胞外聚合物 (EPS) 对酸盐溶解真菌Aspergillus niger的生物活性的影响.
- 确定EPS在不同条件下如何影响微生物生长和酸盐溶解机制.
- 评估A. niger.对营养来源的优先利用情况.
主要方法:
- 从Rhodotorula mucilaginosa中提取EPS,具有不同的无机酸盐含量.
- 在不同的条件下种植Aspergillus niger:含量限制,唯一无机酸盐,唯一EPS,以及联合无机酸盐和EPS.
- 测量细胞壁厚度,微生物生物质,酸度和酸酸酶活性.
主要成果:
- 在限制下,EPS的添加减轻了A. niger细胞壁厚度的增加.
- EPS显著促进了微生物的生长,导致生物质增加了四倍.
- 尼日尔的酸转移了其酸溶解策略,从氧酸分泌到在EPS的存在下促进酸酸酶活动.
- 无机酸和EPS的共存导致了氧酸度的降低和酸酸酶活性的增强,首选使用EPS.
结论:
- 黑色虫根据可用的源调整其酸盐溶解策略.
- 细胞外聚合物物质 (EPS) 优先使用于无机酸盐,由A. niger.
- 在微生物应用中,EPS显示出作为供应的可持续来源的巨大潜力.
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