液体染色学-双重质谱法用于临床定量抗生素的方法
Xingrui He1, Wenqian Zhang1, Feifan Xie1
1Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, China.
Bioanalysis
|July 9, 2025
概括
使用液体染色学-并联质谱法 (LC-MS/MS) 精确的抗生素量化对于个性化医学和打击抗菌素耐药性 (AMR) 至关重要. 本综述详细介绍了LC-MS/MS方法,以优化抗生素治疗和患者的治疗结果.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
- 临床微生物学 临床微生物学
背景情况:
- 准确的抗生素量化对于个性化医疗,优化治疗和解决抗菌素耐药性 (AMR) 是必不可少的.
- 液体染色学-双重质谱法 (LC-MS/MS) 是抗生素量化的黄金标准,因为它的准确性,效率和样本兼容性.
研究的目的:
- 提供LC-MS/MS原则,方法和抗生素量化中的临床应用的全面审查.
- 突出LC-MS/MS在治疗药物监测 (TDM),药理动力学 (PK) 和流行病学研究中的作用.
- 讨论各种抗生素类别分析的挑战和策略.
主要方法:
- 审查关于LC-MS/MS的现有文献,用于抗生素量化.
- 分析原则,方法和临床应用.
- 讨论挑战 (样本准备,矩阵效应) 和针对不同类型抗生素的定制策略.
- 探索新兴趋势,如自动化和高分辨率质谱等.
主要成果:
- 在生物矩阵中,LC-MS/MS对抗生素量化非常有效.
- 需要特定的策略来分析各种类别的抗生素,基于它们的特性.
- 自动化和先进的MS技术正在提高效率和准确性.
- 强有力的质量控制对于可靠的结果至关重要.
结论:
- LC-MS/MS是精准医学和AMR缓解的基石技术.
- 在LC-MS/MS领域的持续创新将进一步加强抗生素治疗和全球卫生工作.
- 解决样本准备和矩阵效应方面的挑战是未来进步的关键.
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