相关实验视频

Updated: Sep 16, 2025

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

ALPK1和STING之间的交叉通话:在先天性免疫激活和人类炎症疾病中的协同轴

Chong-Shan Shi^1,2, Christina Kozycki^2,3,4, Ning-Na Huang¹

¹B-cell Molecular Immunology Section, Laboratory of Immunoregulation and Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

bioRxiv : the preprint server for biology

|July 9, 2025

概括

阿尔法激酶1 (ALPK1) 和干扰素基因刺激器 (STING) 途径参与双向信号传递,放大先天免疫反应. 这种交叉语音对于理解免疫平衡和由异常信号驱动的疾病至关重要.

科学领域:

这是天生的免疫力.
分子信号通道的分子信号通道.
微生物传感器是一种微生物传感器.

背景情况:

阿尔法激酶1 (ALPK1) 感知微生物代谢物并通过TIFA激活NF-κB信号.
功能获取ALPK1突变与干扰素驱动的炎症有关,这表明NF-κB以外的作用.
干扰素基因刺激器 (STING) 途径是核酸感知和免疫反应的核心.

研究的目的:

为了研究ALPK1和STING通路之间的交叉声.
阐明ALPK1信号如何影响STING通路输出.
了解ALPK1-STING通路相互作用的临床影响.

主要方法:

研究了ALPK1激活对STING通路输出的影响,包括LC3B脂化和NLRP3炎症酶激活.
评估ALPK1对eIF2α的信号影响,eIF2α是综合应激反应的一个组成部分.
研究了STING激活对ALPK1表达和TIFA酸化的影响.
分析了ALPK1介导疾病患者的临床数据.

主要成果:

ALPK1激活增强了STING通路输出,包括规范和非规范的反应,如LC3B脂化和NLRP3炎症酶激活.
ALPK1信号激活eIF2α,表明与综合应激反应的联系.
激活STING可相互增加ALPK1蛋白水平和TIFA酸化.
通过ALPK1介导的疾病与过早的内矿化和脑脊液内蛋白升高有关,这表明干扰素失调.

结论:

ALPK1和STING通路表现出双向信号,其中一个的激活会放大另一个.
这种交叉声提供了微生物传感 (ALPK1) 和核酸传感 (STING) 途径之间的机械联系.
这些发现提供了对本质稳定和疾病中的先天性免疫调节的见解,特别是在涉及干扰素失调的条件下.

关键词:

在 ALPK1 中,这是一种自发炎症性自发炎症.在炎症中,一些细胞会发炎.干扰素干扰素干扰素刺痛是一种刺痛.这就是TIFA TIFA.

更多相关视频

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Intravenous Endotoxin Challenge in Healthy Humans: An Experimental Platform to Investigate and Modulate Systemic Inflammation

Published on: May 16, 2016

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Intracellular Signaling Cascades

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...