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相关概念视频

Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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相关实验视频

Updated: Sep 16, 2025

Massively Parallel Reporter Assays in Cultured Mammalian Cells
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使用e2MPRARA同时进行表观基因组分析和监管活动测量.

Zicong Zhang1, Ilias Georgakopoulos-Soares2, Guillaume Bourque1,3,4,5

  • 1Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan.

bioRxiv : the preprint server for biology
|July 9, 2025
PubMed
概括

一种名为e2MPRA的新方法同时分析调节元素,蛋白质结合和表观遗传修饰. 这项技术推动了我们对基因组的理解.

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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相关实验视频

Last Updated: Sep 16, 2025

Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

21.8K
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
13:47

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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科学领域:

  • 基因组学和分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 监管基因组学 监管基因组学

背景情况:

  • 关联调节元素 (CREs) 显著影响表型和疾病.
  • 目前的方法如ChIP-seq,Cut&Tag和ATAC-seq可以识别CREs,但只是描述性的.
  • 需要像MPRA这样的高通量技术来评估功能活动和变异效应.

研究的目的:

  • 开发一种新技术,使得可以同时分析CRE调节功能,TF/辅因子结合和表观基因组修饰.
  • 克服现有方法的局限性,这些方法不能整合功能和表观遗传学分析.
  • 为解剖监管代码及其在疾病中的作用提供一个高通量平台.

主要方法:

  • 开发了丰富,随后进行了表观基因组分析MPRA (e2MPRA),这是基于lentivirus的MPRA技术.
  • 丰富的特定CREs用于基因组集成,其次是有针对性的Cut&Tag或ATAC-seq.
  • 能够同时对数千种CRE和变种的调节活性,蛋白质结合和表观遗传修饰进行高通量分析.

主要成果:

  • 证明了e2MPRA在合成增强剂中剖析TF动图的表观遗传功能的能力.
  • 展示了增强剂序列变异对表观遗传修饰的影响的分析.
  • 验证了对候选CREs的监管活性,蛋白质结合和表观基因组数据的同时评估.

结论:

  • e2MPRA是一种新的技术,用于同时对CRE功能,蛋白相互作用和表观基因组状态进行高通量分析.
  • 这种方法增强了对监管代码,对表观基因组及其影响以及疾病机制的理解.
  • e2MPRA提供了一个强大的工具,用于调查监管法规的修改如何导致人类疾病.