通过氧化还原状态传感器CtBP对肠内分泌细胞命运的代谢控制
Bohdana M Rovenko1,2, Kari Moisio1,2, Cornelia Biehler1,2
1Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki 00790, Finland.
bioRxiv : the preprint server for biology
|July 9, 2025
概括
感知营养的肠内分泌细胞控制生理学. 在Drosophila中,CtBP辅因子将糖代谢与细胞命运联系起来,通过感知细胞氧化还原状态来调节高糖饮食中的能量平衡和生存.
科学领域:
- 细胞生物学 细胞生物学
- 代谢过程中的代谢.
- 内分泌学 在内分泌学.
背景情况:
- 肠内分泌 (EE) 细胞通过感知肠道营养素和分泌激素来调节生物体生理.
- 营养素的可用性通过影响细胞命运的决定和维护来影响EE细胞的丰富性.
- 通过EE细胞感知由糖诱导的细胞内代谢变化来控制其反应的机制仍然不清楚.
研究的目的:
- 研究糖诱导的代谢变化是如何被EE细胞感知到,以调节它们的功能和命运.
- 为了确定分子参与者,将营养感应与细胞命运决定联系在Drosophila EE细胞中.
主要方法:
- 研究了NADH结合转录辅因子C-终端结合蛋白 (CtBP) 在Drosophila幼虫EE细胞中的作用.
- 通过尼古丁胺胺辅因子氧化还原平衡分析CtBP二元化调节,受糖解和酸途径的影响.
- 检查了CtBP与转录因子Prospero的相互作用及其与调节EE细胞命运和大小的基因组标的结合.
主要成果:
- 在Drosophila中,CtBP充当营养感应和EE细胞命运调节之间的关键联系.
- CtBP二分化由细胞的氧化还原状态控制,反映了糖的代谢.
- CtBP与Prospero相互作用,并调节参与Notch和胰岛素/mTOR信号通路的基因,影响EE细胞的命运和大小.
结论:
- 细胞内氧化还原状态的变化是EE细胞功能的指导信号.
- CtBP集成了代谢线索来控制EE细胞的命运和大小,从而调节生物体的平衡.
- 这项研究揭示了一种新的营养感应机制,以及它对内分泌细胞生物学和生物生存的影响.
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