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Updated: Sep 16, 2025

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
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Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

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预测一个自由生活的细菌与聚合-AlphaFold3的蛋白质相互作用格局

Horia Todor, Lili M Kim, Jürgen Jänes

    bioRxiv : the preprint server for biology
    |July 9, 2025
    PubMed
    概括

    我们开发了聚合蛋白与蛋白相互作用 (PPI) 预测,这是一种可扩展的全基因组分析方法. 这种技术准确地预测了数千个PPI,使用的计算资源比以前的方法少得多.

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    Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions06:50

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    Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells08:38

    Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

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    相关概念视频

    Protein Networks02:26

    Protein Networks

    An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
    These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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    Protein-protein Interfaces02:04

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    Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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    Conserved Binding Sites01:49

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    Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
    Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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