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Updated: Sep 16, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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基于DpaZn-纳米聚合物的膜上蛋白质N-酸化的高通量捕获和敏感的现场可视化,功能化逆相N-蛋白阵列

Xiaobo Cao1, Yinhe Han1, Xiuli Wang1

  • 1College of Basic Medical Science, Dalian Medical University, Dalian 116044, China.

Analytical chemistry
|July 9, 2025
PubMed
概括

研究人员开发了一种新的N-蛋白阵列 (RPNPA),以克服研究N-酸化的挑战. 这种方法有效地捕获和检测N-蛋白,使这种关键的翻译后修改能够进行高通量分析.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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