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电驱动的化学细胞外囊穿越底屏障,用于视网母细胞瘤治疗

Haoliang Shi¹, Xuan Qin¹, Xingxiu Jiang²

¹Suzhou Key Laboratory of Nanotechnology and Biomedicine, Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Institute of Functional Nano and Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University; Suzhou 215123, China.

|July 9, 2025

概括

一种电活性微针装置改善了细胞外囊泡 (EV) 通过视网膜屏障传递,用于视网膜母细胞瘤治疗. 这种新的方法增强了EV的向和保留,显示出后端药物递送的巨大潜力.

科学领域:

眼科医生眼科眼科
生物材料科学生物材料科学
纳米技术纳米技术

背景情况:

内部限制膜 (ILM) 对视网膜母细胞瘤的静脉内药物输送构成重大障碍.
细胞外囊泡 (EVs) 对视网膜母细胞瘤治疗有希望,但在穿越生物障碍和向瘤细胞方面面临挑战.

研究的目的:

开发一种电动微针 (e-MN) 装置,用于在ILM中增强化学电动汽车的传输.
提高EVs在视网膜母细胞瘤治疗中的向性和治疗疗效.

主要方法:

电动微针 (e-MN) 装置的设计和制造.
巨细胞和视网母细胞瘤衍生的化学EVs的发展.
在猪,老鼠和子眼睛模型中评估e-MN介导的EV传递.
在体外和体内评估EV向,细胞吸收和治疗效果.

主要成果:

与传统的视内注射相比,e-MN装置显著增加了视网膜中的EV度 (10倍).
化学EVs表明增强了视网膜母细胞瘤细胞向 (Y79细胞吸收增加2倍) 和免疫逃避.
嵌入式光遗传系统在嵌合式EV中,在体外约70%的瘤细胞中诱导了亡.
通过e-MN交付的模拟电动汽车表现出优越的眼睛保留和视觉功能保存.

结论:

电动微针平台为克服ILM障碍提供了一种最小侵入性和有效的策略.
化学EVs与e-MN输送相结合,可增强治疗剂的传输和治疗视网膜母细胞瘤的疗效.
这种方法具有很大的潜力,可以推进后端药物递送应用.

关键词:

电活性的电动活动体.细胞外囊泡中的细胞外囊泡.微针微针是一种微针.在光学遗传学方面,光学遗传学是指光学遗传学.视网膜母细胞瘤 (Retinoblastoma) 是一种

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