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高吞吐量选和Saccharopolyspora spinosa的新陈代谢工程,用于酸盐生产
Jiaxin Du1, Jiale Zhang1, Chen Yang1
1School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.
Biotechnology letters
|July 9, 2025
概括
一种新的体外检测方法加快了产生的spinosad (天然杀虫剂) 菌株的繁殖. 这种方法导致了一种突变菌株,增加了脊柱菌种的产量,以及一种基因工程菌株,产量高出近三倍.
科学领域:
- 微生物学和生物技术
- 农业科学 农业科学
- 生物化学 生化学
背景情况:
- 斯皮诺萨德是一种来自Saccharopolyspora spinosa的类抗生素,是一种有效的,环保的杀虫剂.
- 由于发酵不良和菌株工程挑战的高生产成本限制了spinosad的工业用途.
- 传统的培育种菌株的方法是缓慢而劳动密集的.
研究的目的:
- 开发一种加速体外检测方法,用于培育改良的Saccharopolyspora spinosa菌株.
- 通过高吞吐量查和基因工程来增强脊髓产量.
主要方法:
- 建立了一个 in vitro 检测系统,使用 glycosyltransferase OleD 来检测 pseudoaglycone (PSA),这是一个 spinosad 前体.
- 采用色度反应与糖基化相结合,用于PSA检测.
- 优化了PSA检测系统,用于对S. spinosa突变体的高通量查和随后的基因工程.
主要成果:
- 鉴定出突变菌株DUA15,分别增加了0.80倍和0.66倍的西酸和PSA产量.
- 与原始菌株相比,开发了D15-102工程菌株,实现了比原始菌株的2.9倍增长.
- 证明了体外检测方法在加速菌株改善方面的有效性.
结论:
- 开发的体外检测方法显著加快了生产状菌株的繁殖速度.
- 将高吞吐量查与基因工程相结合,提供了一种可行的策略,以提高种产量.
- 提高酸盐生产效率可能会降低成本并扩大其工业应用.
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