过度表达的ABA-响应转录因子PtrMYBH变异会在Populus中激发角髓体,树叶,根和叶子的发育
Ziqi Liu1, Xiaomeng Fan2, Xuqing Liu1
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
The Plant journal : for cell and molecular biology
|July 9, 2025
概括
这项研究确定了PtrMYBH,这是树中一种对酸敏感的转录因子. 过度表达PtrMYBH会导致显著的生长和发育变化,影响叶子,根和系统的发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物生理学 植物生理学
背景情况:
- 酸 (ABA) 对于植物应激反应和生长调节至关重要.
- 响应ABA的转录因子 (TFs) 在植物发育中起着关键作用,但它们在Populus中的功能尚不清楚.
研究的目的:
- 在Populus中识别和描述ABA上调的转录因子 (TF).
- 调查确定TF,PtrMYBH在调节植物生长和发育中的功能.
主要方法:
- 在Populus中确定ABA上调的TF.
- 在转基因树中过度表达PtrMYBH.
- 对转基因系的形态和发育变化的分析.
- RNA测序 (RNA-seq) 用于分析基因表达变化.
主要成果:
- 过度表达PtrMYBH导致叶子形 (减小尺寸,卷曲) 和改变口腔发育.
- 受影响的根部发育,增加偶然根数,但减少长度.
- 造成了射出角髓系统 (SAM) 和茎分化体 (SDX) 的形态变化.
- RNA-seq揭示了PtrMYBH影响参与细胞增殖和发育途径的基因.
- 转基因菌株对ABA的敏感性增加,表现出叶子受损和早期叶子掉落.
结论:
- PtrMYBH是Populus中生长和发育的关键调节剂.
- 它在植物对酸的反应中起着重要作用.
- 这些发现为理解和利用TFs操纵Populus的增长提供了基础.
更多相关视频
09:31Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding
Published on: September 20, 2024
826
09:54The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
8.9K
相关概念视频
02:19Morphogenesis
28.8K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.8K
03:02Primary and Secondary Growth in Roots and Shoots
58.1K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
58.1K
01:25Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
01:57Adaptations that Reduce Water Loss
26.4K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
26.4K