温度诱导的膜贩运驱动了向大脑输送抗体的过程
Fusheng Du1, Qi Wan1, Oleg O Glebov2
1Institute of Neuroregeneration and Neurorehabilitation, Qingdao University, Qingdao 266071, Shandong, China.
概括
中度发烧会增强细胞吸收机制,包括大脑. 这种由温度引起的膜贩运的增加,特别是依赖激素的内细胞分裂,可能会改善通过血脑屏障的药物输送.
科学领域:
- 细胞生物学 细胞生物学
- 生理学 生理学 生理学
- 神经科学是一个神经科学.
背景情况:
- 发烧和高温症的细胞机制尚未完全理解.
- 严重的发烧 (>40°C) 与血脑屏障 (BBB) 透性增加有关.
- 中度发烧对大脑生理学的影响在很大程度上是未知的.
研究的目的:
- 在膜贩运上研究中度发烧 (38-40°C) 的细胞机制.
- 为了确定温度在调节血脑屏障透性的作用.
- 探索高温症的潜力,以增强药物输送到大脑.
主要方法:
- 使用培养细胞和全身高热度 in vivo 模型.
- 评估液相吸收和选择性货物吸收.
- 研究了克拉特林介导的内细胞分裂,巨细胞分裂和依赖动氨酸的通路的参与.
- 检查了高温症对大脑积累系统性给予的抗体的影响.
主要成果:
- 中度高温 (38-40°C) 诱导在培养细胞中吸收液相.
- 克拉特林介导的内细胞和巨细胞在翻译依赖的方式上调.
- 全身高温症增加了包括大脑在内的各种器官的液相吸收.
- 静脉注射的抗体在高温症期间在大脑中积累,这种效应被激素抑制阻断.
- 过热促进了膜贩运,包括外细胞回收利用.
结论:
- 温度是膜交易的系统调节器.
- 动氨酸依赖性内细胞分裂是控制温度诱导的BBB透性的关键机制.
- 过热是一种潜在的策略,可以促进大脑的生物药物输送.
相关概念视频
01:15Transcytosis of IgG
2.9K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.9K
01:44Introduction to Membrane Traffic
7.4K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.4K
01:26Antibody Actions
1.4K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
1.4K