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通过膜介质器调节SHED诱导的巨细胞极化和可塑性

Azadeh Mohammad-Hasani¹, Saeed Mohammadi¹, Mohsen Saeidi²

¹Stem Cell Research Centre, Golestan University of Medical Sciences, Gorgan, Iran; Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran.

|July 9, 2025

概括

来自人体脱皮叶状牙 (SHED) 的干细胞将炎症性巨细胞重新编程为修复性M2表型. SHED-MSCs降低炎症和氧化应激,突出了它们对相关疾病的治疗潜力.

科学领域:

免疫学免疫学免疫学
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

来自人体脱皮叶状牙 (SHED) 的干细胞影响巨细胞的两极分化.
SHED-MSC与巨细胞相互作用的精确机制需要进一步阐明.

研究的目的:

为了研究SHED-MSCs和THP-1细胞衍生的巨细胞之间的交叉对话.
评估SHED-MSCs对巨细胞可塑性,表型和功能的影响.

主要方法:

使用跨井系统,SHED-MSC与M0和M1极化巨细胞间接共同培养.
分析巨细胞表面标记物,细胞因子分泌,氧化应激标记物和基因表达通过流细胞计和其他测试.

主要成果:

SHED-MSCs诱导了对M2巨细胞表型 (CD206+) 的显著转变.
与SHED-MSC共同培养增加了抗炎细胞因子 (TGFB2,IL-10) 和减少了促炎细胞因子 (TNF-α,IL-12).
SHED-MSCs降低了氧化应激标志物 (NO,MDA) 和增强了抗氧化能力 (TAC,SOD,CAT),以及ARG1上调和IL-6R下调.

结论:

SHED-MSCs具有对膜效应,将炎症性巨细胞重新编程为修复性表型.
SHED-MSCs恢复了巨细胞的氧化还原稳定,这表明它有可能治疗炎症和与氧化压力相关的疾病.

关键词:

免疫调节是一种免疫调节.巨细胞的两极分化这就是SHED-MSCs.

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